Title:
METHODS FOR CULTURING IMMUNE CELLS
Document Type and Number:
WIPO Patent Application WO/2022/109501
Kind Code:
A3
Abstract:
The preset disclosure provides methods of culturing TILs in a medium comprising at least about 30 mM to at least about 100 mM potassium ion. In some aspects, the methods disclosed herein enhance expansion of CD8+ TILs, relative to CD4+ TILs. In some aspects, the methods further increase the number of less-differentiated cells, e.g., less-differentiated TILs, in the population of cells. In some aspects, the methods disclosed herein enrich for tumorreactive, e.g., tumor specific, TILs such that clonal diversity is preserved. In some aspects, the cells, e.g., the TILs, are administered to a subject in need thereof.
Inventors:
VODNALA SUMAN KUMAR (US)
PATEL YOGIN (US)
RESTIFO NICHOLAS P (US)
PATEL YOGIN (US)
RESTIFO NICHOLAS P (US)
Application Number:
PCT/US2021/060667
Publication Date:
July 21, 2022
Filing Date:
November 23, 2021
Export Citation:
Assignee:
LYELL IMMUNOPHARMA INC (US)
International Classes:
C12N5/0783; A61K35/17; A61P35/00
Domestic Patent References:
| WO2019100023A1 | 2019-05-23 | |||
| WO2020096927A1 | 2020-05-14 |
Foreign References:
| US20190374577A1 | 2019-12-12 |
Other References:
VODNALA SUMAN KUMAR ET AL: "T cell stemness and dysfunction in tumors are triggered by a common mechanism", SCIENCE, vol. 363, no. 6434, 29 March 2019 (2019-03-29), US, pages eaau0135, XP055822936, ISSN: 0036-8075, Retrieved from the Internet DOI: 10.1126/science.aau0135
EIL ROBERT ET AL: "Ionic immune suppression within the tumour microenvironment limits T cell effector function", NATURE, NATURE PUBLISHING GROUP UK, LONDON, vol. 537, no. 7621, 14 September 2016 (2016-09-14), pages 539 - 543, XP037446051, ISSN: 0028-0836, [retrieved on 20160914], DOI: 10.1038/NATURE19364
FRANCO FABIEN ET AL: "Metabolic and epigenetic regulation of T-cell exhaustion", NATURE METABOLISM, vol. 2, no. 10, 1 October 2020 (2020-10-01), pages 1001 - 1012, XP055895705, Retrieved from the Internet DOI: 10.1038/s42255-020-00280-9
HAN JICHANG ET AL: "Memory CD8+ T cell responses to cancer", SEMINARS IN IMMUNOLOGY, W.B. SAUNDERS COMPANY, PA, US, vol. 49, 1 June 2020 (2020-06-01), XP086408231, ISSN: 1044-5323, [retrieved on 20201130], DOI: 10.1016/J.SMIM.2020.101435
EIL ROBERT ET AL: "Ionic immune suppression within the tumour microenvironment limits T cell effector function", NATURE, NATURE PUBLISHING GROUP UK, LONDON, vol. 537, no. 7621, 14 September 2016 (2016-09-14), pages 539 - 543, XP037446051, ISSN: 0028-0836, [retrieved on 20160914], DOI: 10.1038/NATURE19364
FRANCO FABIEN ET AL: "Metabolic and epigenetic regulation of T-cell exhaustion", NATURE METABOLISM, vol. 2, no. 10, 1 October 2020 (2020-10-01), pages 1001 - 1012, XP055895705, Retrieved from the Internet
HAN JICHANG ET AL: "Memory CD8+ T cell responses to cancer", SEMINARS IN IMMUNOLOGY, W.B. SAUNDERS COMPANY, PA, US, vol. 49, 1 June 2020 (2020-06-01), XP086408231, ISSN: 1044-5323, [retrieved on 20201130], DOI: 10.1016/J.SMIM.2020.101435
Attorney, Agent or Firm:
KIM, Ji Eun et al. (US)
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