METHODS FOR DETECTING PROTEINS OR BINDING PARTNERS FOR PROTEINS

Title:

METHODS FOR DETECTING PROTEINS OR BINDING PARTNERS FOR PROTEINS

Document Type and Number:

WIPO Patent Application WO/2015/028811

Kind Code:

A3

Abstract:

Provided is a method for the detection of a protein, such as haemoglobin or a metalloprotein, in a test sample, such as faecal sample, the method comprising the steps of: (i) exposing the sample to a surface having a binding moiety and permitting the binding moiety to specifically immobilise the protein in the sample; (ii) permitting the immobilised protein to catalyse a reaction of a first reagent with a second reagent; and (iii) detecting, such as electrochemically detecting, the reaction of the first reagent with the second reagent. The binding moiety may be provided on the surface of an electrode. Also provided is a method for detecting an analyte in a sample, which analyte is a binding partner for a protein, the method comprising the steps of: (i) providing a protein that is immobilised, such as specifically immobilised, to a surface; (ii) exposing the immobilised protein to the analyte in the sample, thereby to bind the analyte to the immobilised protein; (iii) exposing the immobilised protein to a first reagent and a second reagent; and (iv) detecting, such as electrochemically detecting, the reaction of first reagent with the second reagent, wherein the immobilised protein is a catalyst for the reaction of the first reagent with the second reagent in the absence of the analyte, and the binding of the analyte to the immobilised protein alters the catalytic activity of the protein for the reaction of the first reagent with the second reagent. The binding moiety can be an antibody. The first reagent can be tetramethylbenzidine and the second reagent can be hydrogen peroxide.

Inventors:

COOPER JONATHAN (GB)
REBOUD JULIEN (GB)

Application Number:

PCT/GB2014/052620

Publication Date:

April 23, 2015

Filing Date:

August 29, 2014

Export Citation:

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Assignee:

UNIV GLASGOW (GB)

International Classes:

C12Q1/00; B01L3/00; C12Q1/28; G01N27/00; G01N33/543; G01N33/553; G01N33/68; G01N33/72

Domestic Patent References:

WO2010055306A1	2010-05-20
WO2011023949A2	2011-03-03

Foreign References:

US5506114A

1996-04-09

Other References:

DONG-MIN KIM ET AL: "Disposable Amperometric Glycated Hemoglobin Sensor for the Finger Prick Blood Test", ANALYTICAL CHEMISTRY, vol. 85, no. 13, 2 July 2013 (2013-07-02), pages 6536 - 6543, XP055151954, ISSN: 0003-2700, DOI: 10.1021/ac401411y
GREGOIRE HERZOG ET AL: "Electrochemical strategies for the label-free detection of amino acids, peptides and proteins", THE ANALYST, vol. 132, no. 7, 1 July 2007 (2007-07-01), pages 615 - 32, XP055151993, ISSN: 0003-2654, DOI: 10.1039/b701472d