Title:
METHODS FOR DETECTING THROMBOCYTOSIS USING BIOMARKERS
Document Type and Number:
WIPO Patent Application WO/2011/035249
Kind Code:
A3
Abstract:
The present invention relates to a method to determine the gene expression profile of thrombocytotic sensitive genes. The method comprises the following steps; obtaining hematologic samples from subjects in a training set, analyzing the obtained hematologic samples with a microarray, measuring the expression values of each gene on the microarray, performing analysis to identify differentially expressed genes in the training set among three cohorts of thrombocytosis, obtaining hematologic samples from subjects in an independent testing set, and validating the identity of the differentially expressed genes in the independent testing set among the three cohorts of thrombocytosis. The invention also relates to a method to distinguish thrombocytosis cohorts. The method includes the following steps; obtaining a hematologic sample from a subject, determining gene expression of a biomarker subset, analyzing gene expression of the biomarker subset, and classifying the subject into one of three cohorts.
More Like This:
Inventors:
BAHOU WADIE F (US)
GNATENKO DMITRI V (US)
GNATENKO DMITRI V (US)
Application Number:
PCT/US2010/049507
Publication Date:
August 18, 2011
Filing Date:
September 20, 2010
Export Citation:
Assignee:
BAHOU WADIE F (US)
GNATENKO DMITRI V (US)
GNATENKO DMITRI V (US)
International Classes:
C12Q1/68; C12N15/12; C40B40/08
Other References:
PUIGDECANET E. ET AL: "Gene expression profiling distinguishes JAK2V617F-negative from JAK2Y617F-positive patients in essential thrombocythemia", LEUKEMIA, vol. 22, no. 7, 15 May 2008 (2008-05-15), pages 1368 - 1376
KOSARI F. ET AL: "Clear cell renal cell carcinoma: gene expression analyses identify a potential signature for tumor aggressiveness", CLINICAL CANCER RESEARCH, vol. 11, no. 14, 15 July 2005 (2005-07-15), pages 5128 - 5139
DE REYNIES A. ET AL: "Gene expression profiling reveals a new classification of adrenocortical tumors and identifies molecular predictors of malignancy and survival", JOURNAL OF CLINICAL ONCOLOGY, vol. 27, no. 7, 12 January 2009 (2009-01-12), pages 1108 - 1115
WARNAT P. ET AL: "Cross-study analysis of gene expression data for intermediate neuroblastoma identifies two biological subtypes", BMC CANCER, vol. 7, 25 May 2007 (2007-05-25), pages 89
GNATENKO D.V. ET AL: "Class prediction models of thrombocytosis using genetic biomarkers", BLOOD, vol. 115, no. 1, 22 September 2009 (2009-09-22), pages 7 - 14
KOSARI F. ET AL: "Clear cell renal cell carcinoma: gene expression analyses identify a potential signature for tumor aggressiveness", CLINICAL CANCER RESEARCH, vol. 11, no. 14, 15 July 2005 (2005-07-15), pages 5128 - 5139
DE REYNIES A. ET AL: "Gene expression profiling reveals a new classification of adrenocortical tumors and identifies molecular predictors of malignancy and survival", JOURNAL OF CLINICAL ONCOLOGY, vol. 27, no. 7, 12 January 2009 (2009-01-12), pages 1108 - 1115
WARNAT P. ET AL: "Cross-study analysis of gene expression data for intermediate neuroblastoma identifies two biological subtypes", BMC CANCER, vol. 7, 25 May 2007 (2007-05-25), pages 89
GNATENKO D.V. ET AL: "Class prediction models of thrombocytosis using genetic biomarkers", BLOOD, vol. 115, no. 1, 22 September 2009 (2009-09-22), pages 7 - 14
Attorney, Agent or Firm:
GROLZ, Edward W. (Scott Murphy & Presser,400 Garden City Plaza,Suite 30, Garden City New York, US)
Download PDF: