Title:
METHODS FOR DETECTION OF ANTI-CYTOMEGALOVIRUS NEUTRALIZING ANTIBODIES
Document Type and Number:
WIPO Patent Application WO/2013/144722
Kind Code:
A3
Abstract:
The present disclosure provides methods useful for determining levels of HCMV infection in host cells and, by extension, determining levels of neutralizing antibodies present in a sample. The present disclosure encompasses the recognition that HCMV viruses that have a fluorescent moiety permit detection of viral infection (e.g., by assessing fluorescence in cells after contacting the host cell with the virus). In some embodiments, levels of HCMV infection are determined by fluorescence detection where the virus has been preincubated with a test sample (e.g., a serum sample) from a subject. In some embodiments, the subject has been administered a candidate HCMV vaccine.
Inventors:
ANDERSON DAVID E (US)
BOZIC JASMINKA (CA)
ONTSOUKA BARTHELEMY (CA)
BOZIC JASMINKA (CA)
ONTSOUKA BARTHELEMY (CA)
Application Number:
PCT/IB2013/001021
Publication Date:
November 28, 2013
Filing Date:
March 27, 2013
Export Citation:
Assignee:
VARIATION BIOTECHNOLOGIES INC (CA)
International Classes:
C12Q1/70; G01N33/52; G01N33/564
Foreign References:
| US20100267583A1 | 2010-10-21 |
Other References:
SACCOCCIO ET AL.: "Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells", VACCINE, vol. 29, 24 March 2011 (2011-03-24), pages 2705 - 2711, XP028172352
Attorney, Agent or Firm:
NAUMAN, David (World Exchange Plaza 100 Queen Street, Suite 110, Ottawa Ontario K1P 1J9, CA)
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