METHODS FOR DETERMINING NUCLEOTIDE SEQUENCE INFORMATION

Title:

METHODS FOR DETERMINING NUCLEOTIDE SEQUENCE INFORMATION

Document Type and Number:

WIPO Patent Application WO2005066370

Kind Code:

A3

Abstract:

Provided herein, is a nucleic acid sequencing method based on detection of Raman signatures of oligonucleotide probes. Raman signatures of individually captured nucleic acid probes, optionally labeled by a Raman label or a positively charged enhancer, are detected. The sequences of captured probes are used to identify the nucleotide sequences of captured probes and complementary target nucleic acids, which are then aligned and used to obtain nucleic acid sequence information. In another embodiment, a method is provided for determining a nucleotide occurrence at a target nucleotide position of a target nucleic acid, that utilizes binding of the target nucleic acid to a labeled oligonucleotide probe that binds to the target nucleic acid, wherein the labeled oligonucleotide probe includes a first label and a second label, the first label being capable of affecting an optical property of the second label.

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Inventors:

SU XING (US)

Application Number:

PCT/US2004/043730

Publication Date:

November 03, 2005

Filing Date:

December 24, 2004

Export Citation:

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Assignee:

INTEL CORP (US)
SU XING (US)

International Classes:

C12Q1/68; (IPC1-7): C12Q1/68

Domestic Patent References:

WO2003095973A2	2003-11-20
WO2000075373A2	2000-12-14
WO1995009248A1	1995-04-06

Foreign References:

US20030059820A1	2003-03-27
US6174677B1	2001-01-16
US5306403A	1994-04-26
US5002867A	1991-03-26
EP0838528A1	1998-04-29

Other References:

VO-DINH T ET AL: "SURFACE-ENHANCED RAMAN GENE PROBES", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 66, no. 20, 15 October 1994 (1994-10-15), pages 3379 - 3383, XP000196550, ISSN: 0003-2700
D. GRAHAM ET AL., CHEMPHYSCHEM, no. 12, 2001, pages 746 - 748, XP002328954

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