Title:
METHODS FOR ENGINEERING POLYPEPTIDE VARIANTS VIA SOMATIC HYPERMUTATION AND POLYPEPTIDES MADE THEREBY
Document Type and Number:
WIPO Patent Application WO2006053021
Kind Code:
A3
Abstract:
Methods using somatic hypermutation (SHM) for producing polypeptide and nucleic acid variants, and nucleic acids encoding such polypeptide variants are disclosed. Such variants may have desired properties. Also disclosed are novel polypeptides, such as improved fluorescent proteins, produced by the novel methods, and nucleic acids, vectors, and host cells comprising such vectors.
Inventors:
TSIEN ROGER Y (US)
WANG LEI (US)
WANG LEI (US)
Application Number:
PCT/US2005/040545
Publication Date:
September 08, 2006
Filing Date:
November 08, 2005
Export Citation:
Assignee:
UNIV CALIFORNIA (US)
TSIEN ROGER Y (US)
WANG LEI (US)
TSIEN ROGER Y (US)
WANG LEI (US)
International Classes:
C12N15/00; C12N15/09
Domestic Patent References:
| WO2003045304A2 | 2003-06-05 |
Other References:
BACHL ET AL.: "Increased transcription levels induce higher mutation rates in a hypermutatung cell line", JOURNAL OF IMMUNOLOGY, vol. 166, 2001, pages 5051 - 5057, XP003000996
ZHOU ET AL.: "Human activation -induced cytidine deaminase is induce by IL-4 and negatively regulated by CD45: Implication of CD45 as a janus kinase phosphatase in antibody diversification", JOURNAL OF IMMUNOLOGY, vol. 170, 2003, pages 1887 - 1893, XP003000997
ZHOU ET AL.: "Human activation -induced cytidine deaminase is induce by IL-4 and negatively regulated by CD45: Implication of CD45 as a janus kinase phosphatase in antibody diversification", JOURNAL OF IMMUNOLOGY, vol. 170, 2003, pages 1887 - 1893, XP003000997
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