Title:
METHODS FOR ENGINEERING AND USE OF CILIATED ORGANOIDS HAVING NATIVE-LIKE, APICAL-OUT POLARITY
Document Type and Number:
WIPO Patent Application WO/2023/288160
Kind Code:
A3
Abstract:
Methods and materials for making and using apical-out ciliated organoids (e.g., apical-out airway organoids) are provided herein.
More Like This:
Inventors:
REN XI (US)
WIJESEKARA PIYUMI (US)
FARIMANI AMIR BARATI (US)
YADAV PRAKARSH (US)
WIJESEKARA PIYUMI (US)
FARIMANI AMIR BARATI (US)
YADAV PRAKARSH (US)
Application Number:
PCT/US2022/072815
Publication Date:
May 11, 2023
Filing Date:
June 08, 2022
Export Citation:
Assignee:
UNIV CARNEGIE MELLON (US)
International Classes:
C12N5/07; C12N5/00; G01N33/50
Other References:
SALAHUDEEN ET AL.: "Progenitor identification and SARS-CoV-2 infection in human distal lung organoids", NATURE, vol. 588, 25 November 2020 (2020-11-25), pages 670 - 675, XP037589736, [retrieved on 20230117], DOI: 10.1038/s41586-020-3014-1
CHRISTINA E. BARKAUSKAS, CHUNG MEI-I, FIORET BRYAN, GAO XIA, KATSURA HIROAKI, HOGAN BRIGID L. M.: "Lung organoids: current uses and future promise", DEVELOPMENT, THE COMPANY OF BIOLOGISTS LTD., GB, vol. 144, no. 6, 15 March 2017 (2017-03-15), GB , pages 986 - 997, XP055576557, ISSN: 0950-1991, DOI: 10.1242/dev.140103
CHAN L, TIEW P, KOH M, LEE C, TER S, YONG V, CHOTIRMALL S H: "A Novel 'Apical-out' Human Airway Organoid System for the Study of Host- Pathogen Interaction in COPD", AM J RESPIR CRIT CARE MED, vol. 203, 1 May 2021 (2021-05-01), XP093017296
LEEMAN KRISTEN T., PESSINA PATRIZIA, LEE JOO-HYEON, KIM CARLA F.: "Mesenchymal Stem Cells Increase Alveolar Differentiation in Lung Progenitor Organoid Cultures", SCIENTIFIC REPORTS, vol. 9, no. 1, XP093065920, DOI: 10.1038/s41598-019-42819-1
STROULIOS GEORGIOS, BROWN TYLER, MORENI GIULIA, KONDRO DOUGLAS, DEI ALESSANDRO, EAVES ALLEN, LOUIS SHARON, HOU JUAN, CHANG WING, P: "Apical-out airway organoids as a platform for studying viral infections and screening for antiviral drugs", SCIENTIFIC REPORTS, vol. 12, no. 1, XP093065918, DOI: 10.1038/s41598-022-11700-z
CHRISTINA E. BARKAUSKAS, CHUNG MEI-I, FIORET BRYAN, GAO XIA, KATSURA HIROAKI, HOGAN BRIGID L. M.: "Lung organoids: current uses and future promise", DEVELOPMENT, THE COMPANY OF BIOLOGISTS LTD., GB, vol. 144, no. 6, 15 March 2017 (2017-03-15), GB , pages 986 - 997, XP055576557, ISSN: 0950-1991, DOI: 10.1242/dev.140103
CHAN L, TIEW P, KOH M, LEE C, TER S, YONG V, CHOTIRMALL S H: "A Novel 'Apical-out' Human Airway Organoid System for the Study of Host- Pathogen Interaction in COPD", AM J RESPIR CRIT CARE MED, vol. 203, 1 May 2021 (2021-05-01), XP093017296
LEEMAN KRISTEN T., PESSINA PATRIZIA, LEE JOO-HYEON, KIM CARLA F.: "Mesenchymal Stem Cells Increase Alveolar Differentiation in Lung Progenitor Organoid Cultures", SCIENTIFIC REPORTS, vol. 9, no. 1, XP093065920, DOI: 10.1038/s41598-019-42819-1
STROULIOS GEORGIOS, BROWN TYLER, MORENI GIULIA, KONDRO DOUGLAS, DEI ALESSANDRO, EAVES ALLEN, LOUIS SHARON, HOU JUAN, CHANG WING, P: "Apical-out airway organoids as a platform for studying viral infections and screening for antiviral drugs", SCIENTIFIC REPORTS, vol. 12, no. 1, XP093065918, DOI: 10.1038/s41598-022-11700-z
Attorney, Agent or Firm:
KAYTOR, Elizabeth N. et al. (US)
Download PDF: