Title:
METHODS FOR FLUORESCENCE DETECTION THAT MINIMIZES UNDESIRABLE BACKGROUND FLUORESCENCE
Document Type and Number:
WIPO Patent Application WO2003062795
Kind Code:
A3
Abstract:
The present invention provides a method for the excitation of a fluorescent sample and the measurement of the fluorescent emission. The method of the present invention has the advantage of significantly reducing the amount of background fluorescence. The method includes the steps of exciting a sample in a substrate with a beam of light that enters the substrate at an angle less than or equal to 45°C, and preferably, less than or equal to 20°, and then collecting the fluorescent emission form the sample with a lens system which focuses the emitted light onto a CCD for detection. Although the following description of the present invention uses a scanning system using channel plates by way of illustration, the method described herein may also be used with non-scanning systems as well as capillary systems.
Inventors:
REEL RICHARD T (US)
Application Number:
PCT/US2003/000849
Publication Date:
November 13, 2003
Filing Date:
January 13, 2003
Export Citation:
Assignee:
APPLERA CORP (US)
REEL RICHARD T (US)
REEL RICHARD T (US)
International Classes:
G01N21/64; (IPC1-7): H01J65/06
Foreign References:
| US5498324A | 1996-03-12 | |||
| US5424841A | 1995-06-13 | |||
| US5543026A | 1996-08-06 | |||
| US5062942A | 1991-11-05 | |||
| US5591981A | 1997-01-07 | |||
| US5784152A | 1998-07-21 | |||
| US5818582A | 1998-10-06 | |||
| US5834758A | 1998-11-10 | |||
| US6211954B1 | 2001-04-03 | |||
| US6252236B1 | 2001-06-26 | |||
| US6300638B1 | 2001-10-09 |
Other References:
See also references of EP 1479097A4
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