Title:
METHODS FOR HELPER-DEPENDENT ADENOVIRAL VECTOR PRODUCTION
Document Type and Number:
WIPO Patent Application WO2002002788
Kind Code:
A3
Abstract:
The present invention provides methods and materials for the production of helper-dependent adenovirus, such as PAV, at high titers. In one embodiment, the invention comprises methods for producing high titers of helper-dependent adenovirus comprising co-transfecting a cell permissive for production of adenovirus with: a) a helper-dependent adenoviral vector comprising inverted terminal repeats (ITRs) and packaging sequence derived from a first adenoviral serotype, and a transgene of interest flanked by said ITRs; and b) a chimeric, packaging-deficient helper adenovirus which contains adenoviral genes derived from the first adenoviral serotype, packaging sequence derived from a second adenoviral serotype, and ITRs derived from either the first or second adenoviral serotypes; and collecting virions produced thereby.
Inventors:
ARMENTANO DONNA
Application Number:
PCT/US2001/015784
Publication Date:
January 03, 2003
Filing Date:
May 16, 2001
Export Citation:
Assignee:
GENZYME CORP (US)
International Classes:
C12N15/861; (IPC1-7): C12N15/86
Domestic Patent References:
| WO1996013597A2 | 1996-05-09 | |||
| WO1999061638A1 | 1999-12-02 | |||
| WO1999041400A1 | 1999-08-19 | |||
| WO2000046360A1 | 2000-08-10 | |||
| WO2001053504A1 | 2001-07-26 |
Other References:
PARKS R J ET AL: "A HELPER.DEPENDENT ADENOVIRUS VECTOR SYSTEM: REMOVAL OF HELPER VIRUS BY CRE-MEDIATED EXCISION OF THE VIRAL PACKAGING SIGNAL", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 93, no. 24, 26 November 1996 (1996-11-26), pages 13565 - 13570, XP000617948, ISSN: 0027-8424
SANDIG V ET AL: "OPTIMIZATION OF THE HELPER-DEPENDENT ADENOVIRUS SYSTEM FOR PRODUCTION AND POTENCY IN VIVO", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 97, no. 3, 1 February 2000 (2000-02-01), pages 1002 - 1007, XP000985990, ISSN: 0027-8424
SANDIG V ET AL: "OPTIMIZATION OF THE HELPER-DEPENDENT ADENOVIRUS SYSTEM FOR PRODUCTION AND POTENCY IN VIVO", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 97, no. 3, 1 February 2000 (2000-02-01), pages 1002 - 1007, XP000985990, ISSN: 0027-8424
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