METHODS FOR INCREASING PRODUCTION OF 3-METHYL-2-BUTENOL USING FUSION PROTEINS

WIPO Patent Application WO/2013/040210

A3

The invention relates, in part, to nucleic acid constructs, genetically modified host cells and methods employing such constructs and host cells to increase the production of 3-methyl-2-butenol from IPP. Thus, in some aspects, the invention provides a genetically modified host cell transformed with a nucleic acid construct encoding a fusion protein comprising a phosphatase capable of catalyzing the dephosphorylation of dimethylallyl diphosphate (DMAPP) linked to an IPP isomerase capable of converting IPP to DMAPP, wherein the nucleic acid construct is operably linked to a promoter. In some embodiments, the genetically modified host cell15 further comprises a nucleic acid encoding a reductase that is capable of converting 3-methyl-2-butenol to 3-methy-butanol. In some embodiments, the reductase is encoded by a nucleic acid construct introduced into the cell. In some embodiments, the IPP isomerase is a Type I isomerase. In some embodiments, the IPP isomerase is a Type II isomerase. In some embodiments, the host cell is selected from a group oftaxonimcal classes consisting of 20 Escherichia, Enterobacter, Azotobacter, Erwinia, Bacillus, Pseudomonas, Klebsiella, Proteus, Salmonella, Serratia, Shigella, Rhizobia, Vitreoscilla, Synechococcus, Synechocystis, and Paracoccus taxonomical classes. In some embodiments, the host cell is an Escherichia coli cell. In some embodiments, the host cell is a fungal cell, such as a yeast cell. In some embodiments, the yeast cell is a Saccharomyces sp. cell. In some embodiments, the host cell is an algal, insect or mammalian cell line. In some embodiments, the phosphatase is nudB from E. coli. In some embodiments, the IPP isomerase is encoded by an idi gene from E. coli or idil gene from Saccharomyces cerevisiae.

WO/2023/280976	YEAST SINGLE NUCLEOTIDE POLYMORPHISMS FOR INDUSTRIALLY RELEVANT PHENOTYPES
WO/2014/151447	PROCESSES AND SYSTEMS FOR THE PRODUCTION OF FERMENTATIVE ALCOHOLS
WO/2014/037912	METHODS FOR PRODUCTION OF ISOBUTANOL THROUGH NUTRIENT STRESS AND GENETICALLY MODIFIED MICROORGANISMS THEREOF

CHOU HOWARD H (US)
KEASLING JAY D (US)

PCT/US2012/055165

May 15, 2014

September 13, 2012

Click for automatic bibliography generation  

UNIV CALIFORNIA (US)
CHOU HOWARD H (US)
KEASLING JAY D (US)

C12P7/16

US20100205855A1	2010-08-19
US20110046422A1	2011-02-24
US20110160501A1	2011-06-30

LAUPITZ ET AL.: "Stereochemical Studies on the Making and Unmaking of Isopentenyl Diphosphate in Different Biological Systems", CHEMISTRY & BIODIVERSITY, vol. 1, 1 September 2004 (2004-09-01), pages 1367 - 1376

LOCKYER, Jean, M. et al. (2 Embarcadero Center 8th Floo, San Francisco California, US)

View/Download PDF      PDF Help

Previous Patent: SYSTEMS AND METHODS FOR PROSTATE TREATMENT

Next Patent: METHODS OF PREDICTING AND DECREASING THE RISK OF PRE-TERM BIRTH