Title:
METHODS AND KITS FOR QUANTIFYING METABOLITES OR ANALYTES
Document Type and Number:
WIPO Patent Application WO/2004/104221
Kind Code:
A2
Abstract:
Methods and kits for quantifying the amount of a metabolite, such as homocysteine, methionine or cysteine, in a sample are disclosed. This involves contacting the sample with a first enzyme capable of degrading a metabolite into a plurality of reaction products and regenerating the metabolite using a second enzyme which is capable of converting at least one of said reaction product(s), together with an additional substrate or substrates, into said metabolite. This process optionally generates an additional by-product or by-products. After repeating this process, the metabolite is quantified by detecting a level of a reaction product formed by enzyme degradation of said metabolite by said first enzyme, wherein the reaction product is different from the reaction product used in regenerating said metabolite, or detecting a level of said optional by-product produced by said second enzyme or detecting a level of an additional substrate. The method may also involve the initial conversion of an analyte in a sample to a metabolite by contacting the analyte with a reducing agent. The present method further provides a method of quantifying the amount of an analyte in sample by reacting the analyte to produce a metabolite in a cyclic reaction.
Inventors:
COOMBS GRAHAM HERBERT (GB)
MOTTRAM JEREMY CHARLES (GB)
MOTTRAM JEREMY CHARLES (GB)
Application Number:
PCT/GB2004/002227
Publication Date:
December 02, 2004
Filing Date:
May 21, 2004
Export Citation:
Assignee:
UNIV GLASGOW (GB)
COOMBS GRAHAM HERBERT (GB)
MOTTRAM JEREMY CHARLES (GB)
COOMBS GRAHAM HERBERT (GB)
MOTTRAM JEREMY CHARLES (GB)
International Classes:
G01N33/68; (IPC1-7): C12Q1/527; C12Q1/34; G01N33/68
Domestic Patent References:
| WO2003040694A2 | 2003-05-15 |
Foreign References:
| US5885767A | 1999-03-23 | |||
| US6306618B1 | 2001-10-23 |
Other References:
YAMAGATA S: "ROLES OF O-ACETYL-L-HOMOSERINE SULFHYDRYLASES IN MICRO-ORGANISMS" BIOCHIMIE, MASSON, PARIS, FR, vol. 71, no. 11/12, 1989, pages 1125-1143, XP001037839 ISSN: 0300-9084
SHIMIZU^A H ET AL: "Cloning and overexpression of the oah1 gene encoding O-acetyl-l-homoserine sulfhydrylase of Thermus thermophilus HB8 and characterization of the gene product" BIOCHIMICA ET BIOPHYSICA ACTA. PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, ELSEVIER, AMSTERDAM,, NL, vol. 1549, no. 1, 10 September 2001 (2001-09-10), pages 61-72, XP004305697 ISSN: 0167-4838
SHIMIZU^A H ET AL: "Cloning and overexpression of the oah1 gene encoding O-acetyl-l-homoserine sulfhydrylase of Thermus thermophilus HB8 and characterization of the gene product" BIOCHIMICA ET BIOPHYSICA ACTA. PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, ELSEVIER, AMSTERDAM,, NL, vol. 1549, no. 1, 10 September 2001 (2001-09-10), pages 61-72, XP004305697 ISSN: 0167-4838
Attorney, Agent or Firm:
Kiddle, Simon (York House 23 Kingsway, London Greater London WC2B 6HP, GB)
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