Title:
METHODS FOR MAKING EMBRYONIC CELLS, EMBRYOS, AND ANIMALS SENSITIZED TO STRESS
Document Type and Number:
WIPO Patent Application WO/2011/002955
Kind Code:
A3
Abstract:
Embodiments of the invention are based upon the discovery that exposure of cleavage-stage embryos to a stress inducer, e.g. heat shock or chemical, renders the exposed embryos more sensitive to a secondary treatment with a stress inducer, e.g. heat shock or chemical inducer. Accordingly, the present invention is directed to methods for making embryos, embryonic cells arising from them, and animals and plants that are sensitized to stress, e.g. physiologic or chemical stressors. Methods of screening for inducers and inhibitors of stress using, as test model systems, embryonic cells, embryos, animals, and plants that are sensitized to stress are also disclosed.
Inventors:
WANGH LAWRENCE J (US)
HARTSHORN CRISTINA (US)
JIA YANWEI (US)
HARTSHORN CRISTINA (US)
JIA YANWEI (US)
Application Number:
PCT/US2010/040701
Publication Date:
May 05, 2011
Filing Date:
July 01, 2010
Export Citation:
Assignee:
UNIV BRANDEIS (US)
WANGH LAWRENCE J (US)
HARTSHORN CRISTINA (US)
JIA YANWEI (US)
WANGH LAWRENCE J (US)
HARTSHORN CRISTINA (US)
JIA YANWEI (US)
International Classes:
C12N5/073; C12N5/02; C12Q1/02
Other References:
HARTSHORN, C. ET AL.: "Early onset of heat-shock response in mouse embryos re vealed by quantification of stress-inducible hsp70iRNA", GENE REGULATION AND SYSTEMS BIOLOGY, vol. 1, 2007, pages 365 - 373
AL-KATANANI, Y.M. ET AL.: "Induced thermotolerance in bovine two-cell embryos and the role of heat shock protein 70 in embryoinc development", MOLECULAR R EPRODUCTION AND DEVELOPMENT, vol. 62, 2002, pages 174 - 180
KIM, M. ET AL.: "Expression pattern of HSP25 in mouse preimplantation embryo: heat shock responses during oocyte maturation", MOLECULAR REPRODUCTION AND D EVELOPMENT, vol. 61, 2002, pages 3 - 13
JIA, Y. ET AL.: "Heat shock memory in preimplantation mouse embryos", FERTIL. STERIL., vol. 93, no. 8, 7 April 2010 (2010-04-07), pages 2760 - 2763, XP027047257
AL-KATANANI, Y.M. ET AL.: "Induced thermotolerance in bovine two-cell embryos and the role of heat shock protein 70 in embryoinc development", MOLECULAR R EPRODUCTION AND DEVELOPMENT, vol. 62, 2002, pages 174 - 180
KIM, M. ET AL.: "Expression pattern of HSP25 in mouse preimplantation embryo: heat shock responses during oocyte maturation", MOLECULAR REPRODUCTION AND D EVELOPMENT, vol. 61, 2002, pages 3 - 13
JIA, Y. ET AL.: "Heat shock memory in preimplantation mouse embryos", FERTIL. STERIL., vol. 93, no. 8, 7 April 2010 (2010-04-07), pages 2760 - 2763, XP027047257
Attorney, Agent or Firm:
KARTTUNEN, Leena H. et al. (100 Summer StreetBoston, Massachusetts, US)
Download PDF:
Previous Patent: METHOD, SYSTEM AND APPARATUS FOR PROVIDING AUTOMATION MANAGEMENT SERVICES
Next Patent: ALDEHYDE-FUNCTIONALIZED POLYSACCHARIDES
Next Patent: ALDEHYDE-FUNCTIONALIZED POLYSACCHARIDES