METHODS FOR MAKING A TISSUE ENGINEERED MUSCLE REPAIR (TEMR) CONSTRUCT IN VITRO FOR IMPLANTATION IN VIVO

Title:

METHODS FOR MAKING A TISSUE ENGINEERED MUSCLE REPAIR (TEMR) CONSTRUCT IN VITRO FOR IMPLANTATION IN VIVO

Document Type and Number:

WIPO Patent Application WO/2012/021814

Kind Code:

A3

Abstract:

Provided herein are methods of culturing organized skeletal muscle tissue from precursor muscle cells by cyclically stretching and relaxing said muscle cells on a support in vitro for a time sufficient to produce said organized skeletal muscle tissue, including reseeding said organized skeletal muscle tissue by contacting additional precursor muscle cells to said organized skeletal muscle tissue on said solid support, and then repeating said step of cyclically stretching and relaxing said muscle cells in said support in vitro for time sufficient to enhance the density (i.e., increased number of nuclei and/or number of multinucleated cells) of said organized skeletal muscle tissue on said support.

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Inventors:

CHRIST GEORGE J (US)
CORONA BENJAMIN T (US)

Application Number:

PCT/US2011/047600

Publication Date:

April 25, 2013

Filing Date:

August 12, 2011

Export Citation:

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Assignee:

UNIV WAKE FOREST HEALTH SCIENCES (US)
CHRIST GEORGE J (US)
CORONA BENJAMIN T (US)

International Classes:

C12N5/07; A61K35/34; A61K35/12

Foreign References:

US20090265005A1	2009-10-22
US20020115208A1	2002-08-22

Other References: