Title:
METHODS AND MICROORGANISMS FOR PRODUCTION OF C4-DICARBOXYLIC ACIDS
Document Type and Number:
WIPO Patent Application WO/2010/111344
Kind Code:
A3
Abstract:
Methods and recombinant microorganisms for the biological production of C4-dicarboxylic acids are described. The recombinant microorganisms have increased production of OAA as compared to unmodified microorganisms. In some cases, the recombinant microorganisms have been modified to reduce production of pyruvate and/or reduce conversion of pyruvate to acetaldehyde. Thus, the microorganisms are modified to reduce the activity of pyruvate kinase and/or pyruvate decarboxylase.
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Inventors:
LAWRENCE ADAM (US)
PRONK JACOBUS THOMAS (NL)
VAN MARIS ANTONIUS JEROEN ADRIAAN (NL)
ZELLE RINTZE MEINDERT (NL)
MADDEN KEVIN T (US)
HARRISON JACOB C (US)
TRUEHEART JOSHUA (US)
GANCEDO RODRIGUEZ CARLOS (ES)
FLORES MAURIZ CARMEN-LISSET (ES)
BOWER STANLEY (US)
PRONK JACOBUS THOMAS (NL)
VAN MARIS ANTONIUS JEROEN ADRIAAN (NL)
ZELLE RINTZE MEINDERT (NL)
MADDEN KEVIN T (US)
HARRISON JACOB C (US)
TRUEHEART JOSHUA (US)
GANCEDO RODRIGUEZ CARLOS (ES)
FLORES MAURIZ CARMEN-LISSET (ES)
BOWER STANLEY (US)
Application Number:
PCT/US2010/028429
Publication Date:
July 21, 2011
Filing Date:
March 24, 2010
Export Citation:
Assignee:
MICROBIA INC (US)
LAWRENCE ADAM (US)
PRONK JACOBUS THOMAS (NL)
VAN MARIS ANTONIUS JEROEN ADRIAAN (NL)
ZELLE RINTZE MEINDERT (NL)
MADDEN KEVIN T (US)
HARRISON JACOB C (US)
TRUEHEART JOSHUA (US)
GANCEDO RODRIGUEZ CARLOS (ES)
FLORES MAURIZ CARMEN-LISSET (ES)
BOWER STANLEY (US)
LAWRENCE ADAM (US)
PRONK JACOBUS THOMAS (NL)
VAN MARIS ANTONIUS JEROEN ADRIAAN (NL)
ZELLE RINTZE MEINDERT (NL)
MADDEN KEVIN T (US)
HARRISON JACOB C (US)
TRUEHEART JOSHUA (US)
GANCEDO RODRIGUEZ CARLOS (ES)
FLORES MAURIZ CARMEN-LISSET (ES)
BOWER STANLEY (US)
International Classes:
C12N1/15; C12N15/54; C12P7/46; C12R1/66; C12R1/85
Domestic Patent References:
| WO2009011974A1 | 2009-01-22 |
Other References:
HYOHAK SONG ET AL: "Production of succinic acid by bacterial fermentation", ENZYME AND MICROBIAL TECHNOLOGY, vol. 39, no. ISS.3, 2006, pages 352 - 361
HUI WU ET AL: "Improved Succinic Acid Production in the Anaerobic Culture of an Escherichia coli pflB IdhA Double Mutant as a Result of Enhanced Anaplerotic Activities in the Preceding Aerobic Culture", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 73, no. 24, 2007, pages 7837 - 7843
CHRISTIAN RIEDEL ET AL: "Characterization of the Phosphoenolpyruvate Carboxykinase Gene from Corynebacterium glutamicum and Significance of the Enzyme for Growth and Amino Acid Production", J. MOL. MICROBIOL. BIOTECHNOL., vol. 3, no. 4, 2001, pages 573 - 583
SANG JUN LEE ET AL: "Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 72, no. 3, 2006, pages 1939 - 1948
HUI WU ET AL: "Improved Succinic Acid Production in the Anaerobic Culture of an Escherichia coli pflB IdhA Double Mutant as a Result of Enhanced Anaplerotic Activities in the Preceding Aerobic Culture", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 73, no. 24, 2007, pages 7837 - 7843
CHRISTIAN RIEDEL ET AL: "Characterization of the Phosphoenolpyruvate Carboxykinase Gene from Corynebacterium glutamicum and Significance of the Enzyme for Growth and Amino Acid Production", J. MOL. MICROBIOL. BIOTECHNOL., vol. 3, no. 4, 2001, pages 573 - 583
SANG JUN LEE ET AL: "Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 72, no. 3, 2006, pages 1939 - 1948
Attorney, Agent or Firm:
MEIKLEJOHN, Anita, L. (P.O. Box 1022Minneapolis, MN, US)
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