Title:
METHODS FOR MODULATING PROTEIN GLYCOSYLATION PROFILES OF RECOMBINANT PROTEIN THERAPEUTICS USING MONOSACCHARIDES AND OLIGOSACHARIDES
Document Type and Number:
WIPO Patent Application WO/2014/151878
Kind Code:
A3
Abstract:
The present invention relates to the field of protein production, and in particular to methods and compositions for modulating glycosylation of proteins expressed in host cells.
Inventors:
HOSSLER PATRICK (US)
MCDERMOTT SEAN (US)
RACICOT CHRISTOPHER (US)
CORREIA IVAN (US)
MCDERMOTT SEAN (US)
RACICOT CHRISTOPHER (US)
CORREIA IVAN (US)
Application Number:
PCT/US2014/026606
Publication Date:
January 22, 2015
Filing Date:
March 13, 2014
Export Citation:
Assignee:
ABBVIE INC (US)
International Classes:
C07K16/00; C12N5/00; C12P21/00
Domestic Patent References:
| WO2012149197A2 | 2012-11-01 | |||
| WO2004058944A2 | 2004-07-15 |
Other References:
GRAMER M J ET AL: "Modulation of Antibody Galactosylation Through Feeding of Uridine, Manganese Chloride, and Galactose", BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US, vol. 108, no. 7, 1 July 2011 (2011-07-01), pages 1591 - 1602, XP002688515, ISSN: 0006-3592, [retrieved on 20110218], DOI: 10.1002/BIT.23075
NIKI S.C. WONG ET AL: "An investigation of intracellular glycosylation activities in CHO cells: Effects of nucleotide sugar precursor feeding", BIOTECHNOLOGY AND BIOENGINEERING, vol. 107, no. 2, 1 October 2010 (2010-10-01), pages 321 - 336, XP055093757, ISSN: 0006-3592, DOI: 10.1002/bit.22812
GU XUEJUN ET AL: "Improvement of interferon-gamma sialylation in Chinese hamster ovary cell culture by feeding of N-acetylmannosamine", BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US, vol. 58, no. 6, 20 June 1998 (1998-06-20), pages 642 - 648, XP002171407, ISSN: 0006-3592, DOI: 10.1002/(SICI)1097-0290(19980620)58:6<642::AID-BIT10>3.0.CO;2-9
KANDA YUTAKA ET AL: "Comparison of biological activity among nonfucosylated therapeutic IgG1 antibodies with three different N-linked Fc oligosaccharides: the high-mannose, hybrid, and complex types", GLYCOBIOLOGY, OXFORD UNIVERSITY PRESS, US, vol. 17, no. 1, 29 September 2006 (2006-09-29), pages 104 - 118, XP002443679, ISSN: 0959-6658, DOI: 10.1093/GLYCOB/CWL057
GARY WALSH ET AL: "Post-translational modifications in the context of therapeutic proteins", NATURE BIOTECHNOLOGY, vol. 24, no. 10, 1 October 2006 (2006-10-01), pages 1241 - 1252, XP055024914, ISSN: 1087-0156, DOI: 10.1038/nbt1252
EFREN PACIS ET AL: "Effects of cell culture conditions on antibody N-linked glycosylation-what affects high mannose 5 glycoform", BIOTECHNOLOGY AND BIOENGINEERING, vol. 108, no. 10, 1 October 2011 (2011-10-01), pages 2348 - 2358, XP055080567, ISSN: 0006-3592, DOI: 10.1002/bit.23200
NIKI S.C. WONG ET AL: "An investigation of intracellular glycosylation activities in CHO cells: Effects of nucleotide sugar precursor feeding", BIOTECHNOLOGY AND BIOENGINEERING, vol. 107, no. 2, 1 October 2010 (2010-10-01), pages 321 - 336, XP055093757, ISSN: 0006-3592, DOI: 10.1002/bit.22812
GU XUEJUN ET AL: "Improvement of interferon-gamma sialylation in Chinese hamster ovary cell culture by feeding of N-acetylmannosamine", BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US, vol. 58, no. 6, 20 June 1998 (1998-06-20), pages 642 - 648, XP002171407, ISSN: 0006-3592, DOI: 10.1002/(SICI)1097-0290(19980620)58:6<642::AID-BIT10>3.0.CO;2-9
KANDA YUTAKA ET AL: "Comparison of biological activity among nonfucosylated therapeutic IgG1 antibodies with three different N-linked Fc oligosaccharides: the high-mannose, hybrid, and complex types", GLYCOBIOLOGY, OXFORD UNIVERSITY PRESS, US, vol. 17, no. 1, 29 September 2006 (2006-09-29), pages 104 - 118, XP002443679, ISSN: 0959-6658, DOI: 10.1093/GLYCOB/CWL057
GARY WALSH ET AL: "Post-translational modifications in the context of therapeutic proteins", NATURE BIOTECHNOLOGY, vol. 24, no. 10, 1 October 2006 (2006-10-01), pages 1241 - 1252, XP055024914, ISSN: 1087-0156, DOI: 10.1038/nbt1252
EFREN PACIS ET AL: "Effects of cell culture conditions on antibody N-linked glycosylation-what affects high mannose 5 glycoform", BIOTECHNOLOGY AND BIOENGINEERING, vol. 108, no. 10, 1 October 2011 (2011-10-01), pages 2348 - 2358, XP055080567, ISSN: 0006-3592, DOI: 10.1002/bit.23200
Attorney, Agent or Firm:
ZACHARAKIS, Maria Laccotripe et al. (LLP265 Franklin Stree, Boston MA, US)
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