Title:
METHODS OF OIL PRODUCTION IN MICROORGANISMS
Document Type and Number:
WIPO Patent Application WO/2015/177641
Kind Code:
A3
Abstract:
Provided herein are methods for producing one or more polyunsaturated fatty acids. The methods include the steps of providing a microorganism capable of producing polyunsaturated fatty acids, providing a medium comprising a high concentration of one or more carbon sources, low pH, or both, and culturing the microorganism in the medium under sufficient conditions to produce the one or more polyunsaturated fatty acids. Also provided are methods of culturing one or more microorganisms. The methods include culturing the microorganisms in a medium comprising a first amount of one or more carbon sources at a first concentration level, monitoring a carbon source concentration until the carbon source concentration is reduced below the first concentration level, and adding to the medium a second amount of one or more carbon sources to increase the carbon source concentration to a second concentration level.
Inventors:
SUN ZHIYONG (CA)
ARMENTA ROBERTO E (CA)
VALENTINE MERCIA (CA)
ARMENTA ROBERTO E (CA)
VALENTINE MERCIA (CA)
Application Number:
PCT/IB2015/001407
Publication Date:
January 21, 2016
Filing Date:
May 21, 2015
Export Citation:
Assignee:
MARA RENEWABLES CORP (CA)
International Classes:
C12P7/64; C12N1/00; C12N1/10; C12P7/40
Other References:
CHANG ET AL.: "Improvement of docosahexaenoic acid production on glycerol by Schizochytrium sp. S31 with constantly high oxygen transfer coefficient", BIORESOURCE TECHNOLOGY, vol. 142, August 2013 (2013-08-01), pages 400 - 406, XP028576165, ISSN: 0960-8524, DOI: doi:10.1016/j.biortech.2013.04.107
REN ET AL.: "Regulation of docosahexaenoic acid production by Schizochytrium sp.: effect of nitrogen addition", BIOPROCESS AND BIOSYSTEMS ENGINEERING, vol. 37, 21 September 2013 (2013-09-21), pages 865 - 872, XP035315944, ISSN: 1615-7605, DOI: doi:10.1007/s00449-013-1057-5
QU ET AL.: "Batch, fed-batch and repeated fed-batch fermentation processes of the marine thraustochytrid Schizochytrium sp. for producing docosahexaenoic acid", BIOPROCESS AND BIOSYSTEMS ENGINEERING, vol. 36, December 2013 (2013-12-01), pages 1905 - 1912, XP055136084, ISSN: 1615-7591, DOI: doi:10.1007/s00449-013-0966-7
REN ET AL.: "Regulation of docosahexaenoic acid production by Schizochytrium sp.: effect of nitrogen addition", BIOPROCESS AND BIOSYSTEMS ENGINEERING, vol. 37, 21 September 2013 (2013-09-21), pages 865 - 872, XP035315944, ISSN: 1615-7605, DOI: doi:10.1007/s00449-013-1057-5
QU ET AL.: "Batch, fed-batch and repeated fed-batch fermentation processes of the marine thraustochytrid Schizochytrium sp. for producing docosahexaenoic acid", BIOPROCESS AND BIOSYSTEMS ENGINEERING, vol. 36, December 2013 (2013-12-01), pages 1905 - 1912, XP055136084, ISSN: 1615-7591, DOI: doi:10.1007/s00449-013-0966-7
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