Title:
METHODS FOR PREPARATION OF NUCLEIC ACID SEQUENCING LIBRARIES
Document Type and Number:
WIPO Patent Application WO/2019/191459
Kind Code:
A8
Abstract:
Processes and kits are provided for producing sequence specific fragments of nucleic acid molecules, whether from a genome or transcriptome, where one end of the molecule is highly diverse and/or the full-length molecule, whether a gene or a mRNA, is too long for it to be sequenced using currently available sequencing methods. Methods of preparing a sequencing library configured for 5' or 3' anchored sequencing, wherein the opposing termini of the library molecules are differentially truncated, and methods of parallel sequencing such libraries are described.
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Inventors:
RAMENANI RAVI (US)
SMITH DUANE (US)
MCEWEN JASON (US)
SOUMILLON MAGALI (US)
SMITH DUANE (US)
MCEWEN JASON (US)
SOUMILLON MAGALI (US)
Application Number:
PCT/US2019/024623
Publication Date:
October 24, 2019
Filing Date:
March 28, 2019
Export Citation:
Assignee:
BERKELEY LIGHTS INC (US)
International Classes:
C12N15/09; C12N15/10; C12Q1/6806
Attorney, Agent or Firm:
RADEL, Peggy, A. et al. (US)
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