Title:
METHODS OF PRODUCING 7-CARBON CHEMICALS VIA METHYL-ESTER SHIELDED CARBON CHAIN ELONGATION
Document Type and Number:
WIPO Patent Application WO/2014/105794
Kind Code:
A3
Abstract:
This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on enzymes or homologs accepting methyl ester shielded dicarboxylic acid substrates.
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Inventors:
BOTES ADRIANA LEONORA (GB)
CONRADIE ALEX VAN ECK (GB)
CHEN CHANGLIN (GB)
PEARLMAN PAUL S (US)
CONRADIE ALEX VAN ECK (GB)
CHEN CHANGLIN (GB)
PEARLMAN PAUL S (US)
Application Number:
PCT/US2013/077420
Publication Date:
September 12, 2014
Filing Date:
December 23, 2013
Export Citation:
Assignee:
INVISTA NORTH AMERICA SARL (US)
International Classes:
C12P7/18; C12N15/63; C12P7/42; C12P7/44; C12P13/00
Domestic Patent References:
| WO2013003744A2 | 2013-01-03 | |||
| WO2013096898A2 | 2013-06-27 |
Other References:
MADELYN M. SHAPIRO ET AL: "Remarkable Diversity in the Enzymes Catalyzing the Last Step in Synthesis of the Pimelate Moiety of Biotin", PLOS ONE, vol. 7, no. 11, 9 November 2012 (2012-11-09), pages e49440, XP055113391, DOI: 10.1371/journal.pone.0049440
S. LIN ET AL: "The BioC O-Methyltransferase Catalyzes Methyl Esterification of Malonyl-Acyl Carrier Protein, an Essential Step in Biotin Synthesis", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 287, no. 44, 10 September 2012 (2012-09-10), pages 37010 - 37020, XP055113375, ISSN: 0021-9258, DOI: 10.1074/jbc.M112.410290
DAVID C CANTU ET AL: "Thioesterases: A new perspective based on their primary and tertiary structures", PROTEIN SCIENCE,, vol. 19, 1 January 2010 (2010-01-01), pages 1281 - 1295, XP002722509, DOI: 10.1002/PRO.417
JOHN E CRONAN ET AL: "Synthesis of the ,-dicarboxylic acid precursor of biotin by the canonical fatty acid biosynthetic pathway", CURRENT OPINION IN CHEMICAL BIOLOGY, vol. 15, no. 3, 8 March 2011 (2011-03-08), pages 407 - 413, XP028229490, ISSN: 1367-5931, [retrieved on 20110308], DOI: 10.1016/J.CBPA.2011.03.001
STEVEN LIN ET AL: "Closing in on complete pathways of biotin biosynthesis", MOLECULAR BIOSYSTEMS, vol. 7, no. 6, 1 January 2011 (2011-01-01), pages 1811, XP055113373, ISSN: 1742-206X, DOI: 10.1039/c1mb05022b
See also references of EP 2938733A2
S. LIN ET AL: "The BioC O-Methyltransferase Catalyzes Methyl Esterification of Malonyl-Acyl Carrier Protein, an Essential Step in Biotin Synthesis", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 287, no. 44, 10 September 2012 (2012-09-10), pages 37010 - 37020, XP055113375, ISSN: 0021-9258, DOI: 10.1074/jbc.M112.410290
DAVID C CANTU ET AL: "Thioesterases: A new perspective based on their primary and tertiary structures", PROTEIN SCIENCE,, vol. 19, 1 January 2010 (2010-01-01), pages 1281 - 1295, XP002722509, DOI: 10.1002/PRO.417
JOHN E CRONAN ET AL: "Synthesis of the ,-dicarboxylic acid precursor of biotin by the canonical fatty acid biosynthetic pathway", CURRENT OPINION IN CHEMICAL BIOLOGY, vol. 15, no. 3, 8 March 2011 (2011-03-08), pages 407 - 413, XP028229490, ISSN: 1367-5931, [retrieved on 20110308], DOI: 10.1016/J.CBPA.2011.03.001
STEVEN LIN ET AL: "Closing in on complete pathways of biotin biosynthesis", MOLECULAR BIOSYSTEMS, vol. 7, no. 6, 1 January 2011 (2011-01-01), pages 1811, XP055113373, ISSN: 1742-206X, DOI: 10.1039/c1mb05022b
See also references of EP 2938733A2
Attorney, Agent or Firm:
MCCORMICK GRAHAM, Monica et al. (P.O. Box 1022Minneapolis, Minnesota, US)
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