Title:
METHODS FOR PRODUCING A COMPLEX TRANSGENIC TRAIT LOCUS
Document Type and Number:
WIPO Patent Application WO/2012/129373
Kind Code:
A3
Abstract:
Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break- inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
Inventors:
LASSNER MICHAEL (US)
JANTZ DEREK (US)
SMITH JAMES JEFFERSON (US)
CIGAN MARK (US)
FALCO CARL (US)
GAO HUIRONG (US)
LI ZHONGSEN (US)
LIU ZHAN-BIN (US)
SVITASHEV SERGEI (US)
JANTZ DEREK (US)
SMITH JAMES JEFFERSON (US)
CIGAN MARK (US)
FALCO CARL (US)
GAO HUIRONG (US)
LI ZHONGSEN (US)
LIU ZHAN-BIN (US)
SVITASHEV SERGEI (US)
Application Number:
PCT/US2012/030061
Publication Date:
March 14, 2013
Filing Date:
March 22, 2012
Export Citation:
Assignee:
PIONEER HI BRED INT (US)
DU PONT (US)
LASSNER MICHAEL (US)
JANTZ DEREK (US)
SMITH JAMES JEFFERSON (US)
CIGAN MARK (US)
FALCO CARL (US)
GAO HUIRONG (US)
LI ZHONGSEN (US)
LIU ZHAN-BIN (US)
SVITASHEV SERGEI (US)
DU PONT (US)
LASSNER MICHAEL (US)
JANTZ DEREK (US)
SMITH JAMES JEFFERSON (US)
CIGAN MARK (US)
FALCO CARL (US)
GAO HUIRONG (US)
LI ZHONGSEN (US)
LIU ZHAN-BIN (US)
SVITASHEV SERGEI (US)
International Classes:
C12N15/82; A01H1/06; A01H5/10; C12N15/55
Domestic Patent References:
| WO2009042164A1 | 2009-04-02 | |||
| WO2009114321A2 | 2009-09-17 | |||
| WO2010077319A1 | 2010-07-08 | |||
| WO2008148559A1 | 2008-12-11 | |||
| WO2011117249A1 | 2011-09-29 | |||
| WO2009006297A2 | 2009-01-08 |
Other References:
TOWNSEND JEFFREY A ET AL: "High-frequency modification of plant genes using engineered zinc-finger nucleases", NATURE: INTERNATIONAL WEEKLY JOURNAL OF SCIENCE, NATURE PUBLISHING GROUP, UNITED KINGDOM, vol. 459, no. 7245, 21 May 2009 (2009-05-21), pages 442 - 445, XP002588972, ISSN: 0028-0836, DOI: 10.1038/NATURE07845
QUE Q., ET AL.: "Trait stacking in transgenic crops: challenges and opportunities", GM CROPS, vol. 1, no. 4, October 2010 (2010-10-01), pages 220 - 229, XP002677400
QUE Q., ET AL.: "Trait stacking in transgenic crops: challenges and opportunities", GM CROPS, vol. 1, no. 4, October 2010 (2010-10-01), pages 220 - 229, XP002677400
Attorney, Agent or Firm:
ATKINSON, William, M. et al. (Bank Of America Plaza101 South Tryon Street, Suite 400, Charlotte NC, US)
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