Title:
METHODS FOR PROTEOME LABELING
Document Type and Number:
WIPO Patent Application WO/2019/217727
Kind Code:
A3
Abstract:
Provided herein are methods for labeling the proteomes of cells, as well as methods for labeling proteins or populations of proteins produced by cells. In some embodiments, the methods comprise introducing variant aminoacyl-tRNA synthetases and noncanonical amino acids into cells. Also provided herein are polynucleotides encoding variant aminoacyl-tRNA synthetases that recognize noncanonical amino acids. The methods and compositions provided herein are useful for, among other things, identifying target cells and identifying biomarkers of interest.
Inventors:
YANG ANDREW (US)
WYSS-CORAY ANTON (US)
BREWER KYLE (US)
WYSS-CORAY ANTON (US)
BREWER KYLE (US)
Application Number:
PCT/US2019/031587
Publication Date:
December 26, 2019
Filing Date:
May 09, 2019
Export Citation:
Assignee:
UNIV LELAND STANFORD JUNIOR (US)
U S GOVERNMENT REPRESENTED BY THE DEPT OF VETERANS AFFAIRS (US)
U S GOVERNMENT REPRESENTED BY THE DEPT OF VETERANS AFFAIRS (US)
International Classes:
C12N15/11; C07K14/00; C12N15/09; G01N33/50; G01N33/58; G01N33/68
Domestic Patent References:
| WO2006135096A1 | 2006-12-21 |
Other References:
MAHDAVI ET AL.: "Engineered Aminoacyl-tRNA Synthetase for Cell -Selective Analysis of Mammalian Protein Synthesis", J AMER CHEM SOC, vol. 138, no. 13, 6 April 2016 (2016-04-06), pages 4278 - 4281
YOKOGAWA ET AL.: "Incorporation of 3-azidotyrosine into proteins through engineering yeast tyrosyl- tRNA synthetase and its application to site-selective protein modification", METHODS MOL BIOL, vol. 607, 2010, pages 227 - 242
ALVAREZ-CASTELAO ET AL.: "Cell-type-specific metabolic labeling of nascent proteomes in vivo", NAT BIOTECHNOL, vol. 35, no. 12, December 2017 (2017-12-01), pages 1196 - 1201, XP055668467
YOKOGAWA ET AL.: "Incorporation of 3-azidotyrosine into proteins through engineering yeast tyrosyl- tRNA synthetase and its application to site-selective protein modification", METHODS MOL BIOL, vol. 607, 2010, pages 227 - 242
ALVAREZ-CASTELAO ET AL.: "Cell-type-specific metabolic labeling of nascent proteomes in vivo", NAT BIOTECHNOL, vol. 35, no. 12, December 2017 (2017-12-01), pages 1196 - 1201, XP055668467
Attorney, Agent or Firm:
HAO, Joe C. et al. (US)
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