Title:
METHODS FOR PURIFYING RECOMBINANT POLYPEPTIDES
Document Type and Number:
WIPO Patent Application WO/2024/017826
Kind Code:
A3
Abstract:
The present invention is directed to a novel process for purifying a recombinant polypeptide from a solution containing one or more impurities. The process may include a chromatography process which includes a wash buffer containing a small ionic radius cation and a large ionic radius anion.
Inventors:
LEVY NICHOLAS E (US)
TERFLOTH GERALD J (US)
TERFLOTH GERALD J (US)
Application Number:
PCT/EP2023/069769
Publication Date:
March 21, 2024
Filing Date:
July 17, 2023
Export Citation:
Assignee:
GLAXOSMITHKLINE IP DEV LTD (GB)
International Classes:
C07K1/16; C07K1/22; C07K16/00
Foreign References:
| EP3728288B1 | 2022-01-19 | |||
| US20130197197A1 | 2013-08-01 | |||
| US20160108084A1 | 2016-04-21 | |||
| US20200048617A1 | 2020-02-13 |
Other References:
EVANS T L ET AL: "Recombinant bacterial expression and purification of human fragile X mental retardation protein isoform 1", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 74, no. 2, 1 December 2010 (2010-12-01), pages 242 - 247, XP027394797, ISSN: 1046-5928, [retrieved on 20100610], DOI: 10.1016/J.PEP.2010.06.002
LANG PETER F ET AL: "Ionic radii for Group 1 and Group 2 halide, hydride, fluoride, oxide, sulfide, selenide and telluride crystals", DALTON TRANSACTIONS, vol. 39, no. 33, 1 January 2010 (2010-01-01), Cambridge, pages 7786 - 7791, XP093090979, ISSN: 1477-9226, Retrieved from the Internet DOI: 10.1039/c0dt00401d
SHANNON R D: "Revised effective ionic radii and systematic studies of interatomic distances in halides and chalcogenides", ACTA CRYSTALLOGR A, vol. 32, no. 5, 1 September 1976 (1976-09-01), pages 751 - 767, XP093090998, ISSN: 0567-7394, DOI: 10.1107/S0567739476001551
MOHAMED ISMAIL ET AL: "Flavin-Dependent Halogenases from Xanthomonas campestris pv. campestris B100 Prefer Bromination over Chlorination", ADVANCED SYNTHESIS AND CATALYSIS, JOHN WILEY & SONS, INC, HOBOKEN, USA, vol. 361, no. 11, 12 March 2019 (2019-03-12), pages 2475 - 2486, XP072359600, ISSN: 1615-4150, DOI: 10.1002/ADSC.201801591
LANG PETER F ET AL: "Ionic radii for Group 1 and Group 2 halide, hydride, fluoride, oxide, sulfide, selenide and telluride crystals", DALTON TRANSACTIONS, vol. 39, no. 33, 1 January 2010 (2010-01-01), Cambridge, pages 7786 - 7791, XP093090979, ISSN: 1477-9226, Retrieved from the Internet
SHANNON R D: "Revised effective ionic radii and systematic studies of interatomic distances in halides and chalcogenides", ACTA CRYSTALLOGR A, vol. 32, no. 5, 1 September 1976 (1976-09-01), pages 751 - 767, XP093090998, ISSN: 0567-7394, DOI: 10.1107/S0567739476001551
MOHAMED ISMAIL ET AL: "Flavin-Dependent Halogenases from Xanthomonas campestris pv. campestris B100 Prefer Bromination over Chlorination", ADVANCED SYNTHESIS AND CATALYSIS, JOHN WILEY & SONS, INC, HOBOKEN, USA, vol. 361, no. 11, 12 March 2019 (2019-03-12), pages 2475 - 2486, XP072359600, ISSN: 1615-4150, DOI: 10.1002/ADSC.201801591
Attorney, Agent or Firm:
KIM, Young-In (GB)
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