Title:
METHODS FOR RAPID DNA EXTRACTION FROM TISSUE AND LIBRARY PREPARATION FOR NANOPORE-BASED SEQUENCING
Document Type and Number:
WIPO Patent Application WO/2020/181191
Kind Code:
A3
Abstract:
The present disclosure relates to improved methods for preparing, sequencing and analyzing short DNA fragments using handheld, nanopore-based sequencing technology as well as improved methods for extracting DNA, in particular genomic DNA, for any downstream application.
Inventors:
WILLIAMS SAMUEL ZEV (US)
WEI SHAN (US)
WEI SHAN (US)
Application Number:
PCT/US2020/021395
Publication Date:
October 22, 2020
Filing Date:
March 06, 2020
Export Citation:
Assignee:
UNIV COLUMBIA (US)
International Classes:
C07H1/00; C12N15/10; C07H21/00; C07H21/04; C12N15/11; C12Q1/68
Foreign References:
| US20180087050A1 | 2018-03-29 | |||
| US20090023180A1 | 2009-01-22 | |||
| US20070122391A1 | 2007-05-31 |
Other References:
SHAN WEI, WEISS ZACHARY R., WILLIAMS ZEV: "Rapid Multiplex Small DNA Sequencing on the MinION Nanopore Sequencing Platform", GENES GENOMES GENETICS, vol. 8, May 2018 (2018-05-01), pages 1649 - 1657, XP055749313
ANONYMOUS: "INSTRUCTION MANUAL NEBNext ® Ultra™ II End Repair/dA-Tailing Module NEB #E7546S/L", NEW ENGLAND BIOLABS INC., March 2020 (2020-03-01), pages 1 - 3, XP055749314, Retrieved from the Internet [retrieved on 20200612]
ANONYMOUS: "INSTRUCTION MANUAL NEBNext ® Ultra™ II End Repair/dA-Tailing Module NEB #E7546S/L", NEW ENGLAND BIOLABS INC., March 2020 (2020-03-01), pages 1 - 3, XP055749314, Retrieved from the Internet
Attorney, Agent or Firm:
CARNEY, Bonnie Kramer (US)
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