Title:
METHODS OF SEARCHING FOR CRYSTALLIZATION CONDITIONS OF BIOPOLYMER AND SEARCHING APPARATUS
Document Type and Number:
WIPO Patent Application WO/2004/019008
Kind Code:
A1
Abstract:
Methods of searching for the crystallization conditions of a biopolymer. Namely, the crystallization conditions of a biopolymer are searched for by adding, to a solution separated from another solution containing the biopolymer by a semipermeable membrane, a substance altering the physicochemical properties of the solution, thus altering the physicochemical properties of the biopolymer-containing solution and then monitoring the state of the biopolymer-containing solution. Further, the crystallization conditions of a biopolymer are searched for by decreasing or increasing the volume of a solution containing the biopolymer separated from another solution by a semipermeable membrane to thereby altering the concentration of the biopolymer in the biopolymer-containing solution and then monitoring the state of the biopolymer-containing solution. A method of producing biopolymer crystals by using the crystallization conditions searched for the above methods. An apparatus for searching for the crystallization conditions of a biopolymer which has a dialytic crystallization chamber and a solution-mixing chamber separated therefrom by a movable semipermeable membrane. The dialytic crystallization chamber has a window for monitoring the inside of the chamber while the solution-mixing chamber is connected to one or more reagent bottles in such a manner that a solution can be supplied from a reagent bottle.
Inventors:
MIYATAKE HIDEYUKI (JP)
TAKEDA KAZUKI (JP)
MIKI KUNIO (JP)
TAKEDA KAZUKI (JP)
MIKI KUNIO (JP)
Application Number:
PCT/JP2003/008765
Publication Date:
March 04, 2004
Filing Date:
July 10, 2003
Export Citation:
Assignee:
RIKEN (JP)
MIYATAKE HIDEYUKI (JP)
TAKEDA KAZUKI (JP)
MIKI KUNIO (JP)
MIYATAKE HIDEYUKI (JP)
TAKEDA KAZUKI (JP)
MIKI KUNIO (JP)
International Classes:
G01N1/28; B01D9/02; C30B7/00; C30B29/58; G01N1/34; G01N21/49; G01N21/51; (IPC1-7): G01N1/28; B01D9/02; C30B29/58; G01N21/49
Foreign References:
| JPH0560665A | 1993-03-12 | |||
| US4833233A | 1989-05-23 | |||
| JPH0524119B2 | 1993-04-06 |
Attorney, Agent or Firm:
SIKS & CO. (Kyobashi-Nisshoku Bldg. 8-7, Kyobashi 1-chom, Chuo-ku Tokyo, JP)
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