Title:
METHODS AND SYSTEMS FOR NUCLEIC ACID SEQUENCING VALIDATION, CALIBRATION AND NORMALIZATION
Document Type and Number:
WIPO Patent Application WO/2011/103467
Kind Code:
A3
Abstract:
A system for performing quality control for nucleic acid sample sequencing is disclosed. The system comprises a set of solid supports, each solid support having attached thereto a plurality of nucleic acid sequences, wherein the set comprises plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived, and the nucleic acids sequences are designed such that the nucleic acid sequences produce a predefined pattern of detectable signals during a sequencing run. A method of preparing a quality control for performing nucleic acid sample sequencing, a method of validating a nucleic acid sequencing instrument during a nucleic acid sequencing experiment, and a method of processing nucleic acid sequencing data during a nucleic acid sequencing experiment are also disclosed.
Inventors:
SHEN MIN-YI (US)
GREINER DOUGLAS (US)
GREINER DOUGLAS (US)
Application Number:
PCT/US2011/025486
Publication Date:
January 05, 2012
Filing Date:
February 18, 2011
Export Citation:
Assignee:
LIFE TECHNOLOGIES CORP (US)
SHEN MIN-YI (US)
GREINER DOUGLAS (US)
SHEN MIN-YI (US)
GREINER DOUGLAS (US)
International Classes:
C12Q1/68; C12N15/11; G16B30/00
Foreign References:
| US20060121491A1 | 2006-06-08 | |||
| US20090181385A1 | 2009-07-16 | |||
| US20080262747A1 | 2008-10-23 | |||
| US20080120079A1 | 2008-05-22 |
Other References:
BINNECK, E. ET AL.: "VSQual: a visual system to assist DNA sequencing quality control", GENET. MOL. RES., vol. 3, no. 4, 30 December 2004 (2004-12-30), pages 474 - 482
Attorney, Agent or Firm:
SCHELL, David et al. (Attention: IP Department5791 Van Allen Wa, Carlsbad CA, US)
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