Title:
METHODS AND SYSTEMS FOR SPORES DETECTION
Document Type and Number:
WIPO Patent Application WO/2012/122561
Kind Code:
A3
Abstract:
Provided herein are methods and systems for the detection of spores in a sample which comprise permeabilizing a protein-based spore coat with a protein degrading agent comprising a non-ionic detergent and in particular with a specific mixture of various protein degrading agents comprising a non-ionic detergent to allow contact of spore nucleic acids with fluorescent reagents suitable for detection.
Inventors:
LA DUC MYRON T (US)
VENKATESWARAN KASTHURI J (US)
MOHAPATRA BIDYUT (US)
VENKATESWARAN KASTHURI J (US)
MOHAPATRA BIDYUT (US)
Application Number:
PCT/US2012/028663
Publication Date:
December 27, 2012
Filing Date:
March 10, 2012
Export Citation:
Assignee:
CALIFORNIA INST OF TECHN (US)
LA DUC MYRON T (US)
VENKATESWARAN KASTHURI J (US)
MOHAPATRA BIDYUT (US)
LA DUC MYRON T (US)
VENKATESWARAN KASTHURI J (US)
MOHAPATRA BIDYUT (US)
International Classes:
C12Q1/04; C12Q1/37; C12Q1/68
Foreign References:
| US20060204978A1 | 2006-09-14 |
Other References:
RAWSTHORNE, H. ET AL.: "PCR-based method using propidium monoazide to distinguish viable from nonviable Bacillus sbtilis spores", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 75, no. 9, May 2009 (2009-05-01), pages 2936 - 2939
HERZOG, A.: "Current detection methods for biological agents of concern", MMG 445 BASIC BIOTECHNOLOGY, vol. 5, 2009, pages 50 - 59
ALMEIDA, J. L. ET AL.: "Requirements for the development of Bacillus Anthracis spore reference materials used to test detection systems", JOURNAL OF RESEARCH OF THE NATIONAL INSTITUTE OF STANDARD AND TECHNOLOGY, vol. 111, no. 3, May 2006 (2006-05-01), pages 205 - 217
HERZOG, A.: "Current detection methods for biological agents of concern", MMG 445 BASIC BIOTECHNOLOGY, vol. 5, 2009, pages 50 - 59
ALMEIDA, J. L. ET AL.: "Requirements for the development of Bacillus Anthracis spore reference materials used to test detection systems", JOURNAL OF RESEARCH OF THE NATIONAL INSTITUTE OF STANDARD AND TECHNOLOGY, vol. 111, no. 3, May 2006 (2006-05-01), pages 205 - 217
Attorney, Agent or Firm:
STEINFL & BRUNO LLP et al. (Suite 700Pasadena, California, US)
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