Title:
METHODS OF USING OLIGONUCLEOTIDE-GUIDED ARGONAUTE PROTEINS
Document Type and Number:
WIPO Patent Application WO/2016/161375
Kind Code:
A3
Abstract:
The invention relates to the use of Argonaute polypeptide:guide molecule complexes as fast and specific nucleic acid probes, as specific, nucleic acid-guided restriction enzymes for DNA and RNA substrates, and as a means to detect RNA-protein interactions, RNA detection, DNA detection, and RNA depletion. Using such Argonaute polypeptide:guide molecule complexes enables fast and specific detection, purification, and enzymatic activity.
Inventors:
ZAMORE PHILLIP DAVID (US)
MOORE MELISSA JEANNE (US)
JOLLY SAMSON MICHAEL (US)
SALOMON WILLIAM EDWARD (US)
SEREBROV VICTOR (US)
ZHANG HAN (CN)
MOORE MELISSA JEANNE (US)
JOLLY SAMSON MICHAEL (US)
SALOMON WILLIAM EDWARD (US)
SEREBROV VICTOR (US)
ZHANG HAN (CN)
Application Number:
PCT/US2016/025724
Publication Date:
November 10, 2016
Filing Date:
April 01, 2016
Export Citation:
Assignee:
UNIV MASSACHUSETTS (US)
International Classes:
C12N15/10
Domestic Patent References:
| WO2014189628A1 | 2014-11-27 |
Other References:
DAAN C. SWARTS ET AL: "DNA-guided DNA interference by a prokaryotic Argonaute", NATURE, vol. 507, no. 7491, 16 February 2014 (2014-02-16), pages 258 - 261, XP055156328, ISSN: 0028-0836, DOI: 10.1038/nature12971
G. SHENG ET AL: "Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 111, no. 2, 27 December 2013 (2013-12-27), pages 652 - 657, XP055156327, ISSN: 0027-8424, DOI: 10.1073/pnas.1321032111
DAAN C SWARTS ET AL: "The evolutionary journey of Argonaute proteins", NATURE STRUCTURAL AND MOLECULAR BIOLOGY, vol. 21, no. 9, 5 September 2014 (2014-09-05), US, pages 743 - 753, XP055287457, ISSN: 1545-9993, DOI: 10.1038/nsmb.2879
G. SHENG ET AL: "Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 111, no. 2, 27 December 2013 (2013-12-27), pages 652 - 657, XP055156327, ISSN: 0027-8424, DOI: 10.1073/pnas.1321032111
DAAN C SWARTS ET AL: "The evolutionary journey of Argonaute proteins", NATURE STRUCTURAL AND MOLECULAR BIOLOGY, vol. 21, no. 9, 5 September 2014 (2014-09-05), US, pages 743 - 753, XP055287457, ISSN: 1545-9993, DOI: 10.1038/nsmb.2879
Attorney, Agent or Firm:
VELEMA, James, H. et al. (28 State StreetBoston, MA, US)
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