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Title:
MICELLAR SPHERICAL NUCLEIC ACIDS FROM THERMORESPONSIVE, TRACELESS TEMPLATES
Document Type and Number:
WIPO Patent Application WO/2018/039629
Kind Code:
A3
Abstract:
Methods of preparing spherical nucleic acids (SNAs) using thermoresponsive traceless templates, such as polyethyleneoxide- polypropyleneoxide-polyethyleneoxide (PEO-PPO-PEO) block copolymers are disclosed. Specifically, the disclosure provides a method comprising: admixing a PEO-PPO-PEO block copolymer and a plurality of amphiphilic oligonucleotides in a buffer to form a SNA, wherein the amphiphilic oligonucleotide comprises (i) a lipid moiety and (ii) at least one reactive group: and admixing the resulting SNA with a PEGylated crosslinking agent to form the crosslinked micellar SNA.

Inventors:
MIRKIN CHAD (US)
NGUYEN SONBINH (US)
BANGA RESHAM (US)
Application Number:
PCT/US2017/048726
Publication Date:
April 25, 2019
Filing Date:
August 25, 2017
Export Citation:
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Assignee:
UNIV NORTHWESTERN (US)
International Classes:
A61K31/00; C07H21/04; C08G63/48; C08G69/48
Foreign References:
US20130136714A12013-05-30
Other References:
BANGA ET AL.: "Liposomal Spherical Nucleic Acids", J AM CHEM SOC., vol. 136, 2014, pages 9866 - 9869, XP055427214, DOI: doi:10.1021/ja504845f
BANERJEE ET AL.: "Poly(ethylene glycol)-Prodrug Conjugates: Concept, Design, and Applications", J DRUG DELIV., vol. 2012, 103973, 2012, pages 1 - 18, XP055079947
TRONG ET AL.: "Mechanisms of micellization and rheology of PEO-PPO-PEO triblock copolymers with various architectures", J COLLOID INTERFACE SCI., vol. 328, no. 2, 2008, pages 278 - 287, XP025611669
CARSON ET AL.: "Hydroxymethyluracil modifications enhance the flexibility and hydrophilicity of double-stranded DNA", NUCLEIC ACIDS RES., vol. 44, no. 5, 17 November 2015 (2015-11-17), pages 2085 - 2092, XP055594882
AISSAOUI ET AL.: "Efficient topical delivery of plasmid DNA to lung in vivo mediated by putativetriggered, PEGylated pDNA nanoparticles", J CONTROL RELEASE, vol. 154, no. 3, 2011, pages 275 - 84, XP028285878, DOI: doi:10.1016/j.jconrel.2011.06.017
BAE ET AL.: "Oil-encapsulating PEO-PPO-PEO/PEG shell cross-linked nanocapsules for target- specific delivery of paclitaxel", BIOMACROMOLECULES, vol. 8, no. 2, 2007, pages 650 - 656, XP055594883
BANGA ET AL.: "Cross-Linked Micellar Spherical Nucleic Acids from Thermoresponsive Templates", J AM CHEM SOC., vol. 139, no. 12, 20 March 2017 (2017-03-20), pages 4278 - 4281, XP055594885
Attorney, Agent or Firm:
DEFOE, Jacqueline J. et al. (US)
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