Title:
MICROALGA-DERIVED EXOSOME MIMETIC AND MANUFACTURING METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2023/008787
Kind Code:
A1
Abstract:
Disclosed herein are a microalga-derived exosome mimetic and a manufacturing method therefor. The method for manufacturing an exosome mimetic of the present invention comprises a step of exclusion processing a buffer solution containing microalgae to prepare nanovesicles. The present invention has the advantage of being able to mass-produce exosome mimetics derived from microalgae on the basis of an extrusion processing method. In particular, the present invention can increase the yield of vesicle production of exosome mimetics derived from microalgae by selectively applying a biolipid surfactant that enhances extrusion processing efficiency.
Inventors:
KIM JIN WOONG (KR)
BAEK HWI RA (KR)
KANG JEONG YI (KR)
KO YU RI (KR)
CHO JUNG HYEON (KR)
SHIN KYOUNG HEE (KR)
KIM JUN OH (KR)
KIM YOUNG SEOK (KR)
BAEK HWI RA (KR)
KANG JEONG YI (KR)
KO YU RI (KR)
CHO JUNG HYEON (KR)
SHIN KYOUNG HEE (KR)
KIM JUN OH (KR)
KIM YOUNG SEOK (KR)
Application Number:
PCT/KR2022/010227
Publication Date:
February 02, 2023
Filing Date:
July 13, 2022
Export Citation:
Assignee:
RESEARCH ＆ BUSINESS FOUNDATION SUNGKYUNKWAN UNIV (KR)
SHINSEGAE INT INC (KR)
SHINSEGAE INT INC (KR)
International Classes:
C12N1/12; A61K8/99; A61Q19/00
Domestic Patent References:
| WO2021122880A1 | 2021-06-24 |
Other References:
ADAMO GIORGIA, FIERLI DAVID, ROMANCINO DANIELE P., PICCIOTTO SABRINA, BARONE MARIA E., ARANYOS ANITA, BOŽIČ DARJA, MORSBACH SVENJA: "Nanoalgosomes: Introducing extracellular vesicles produced by microalgae", JOURNAL OF EXTRACELLULAR VESICLES, vol. 10, no. 6, 1 April 2021 (2021-04-01), UK , pages 1 - 22, XP093031171, ISSN: 2001-3078, DOI: 10.1002/jev2.12081
KANG, Jeong Yi et al. Facile and Large-scale fabrication of exosome-analogous nanovesicles by extrusion of cells with novel cell membrane detergent. 창립 30주년 기념 2020 한국공업화학회 학술대회 연구논문 초록 (Abstracts Presented at 2020 KSIEC Annual Meeting in Celebration of the 30th Anniversary). 2020, pp. 393-394, 3P-486.
CHO JUNG HYEON, KANG JEONG YI, KIM SEULGI, BAEK HWI RA, KIM JUNOH, JANG KWANG-SUK, KIM JIN WOONG: "Skin protein-derived peptide-conjugated vesicular nanocargos for selected skin cell targeting and consequent activation", JOURNAL OF MATERIALS CHEMISTRY. B, vol. 9, no. 24, 23 June 2021 (2021-06-23), GB , pages 4956 - 4962, XP093031174, ISSN: 2050-750X, DOI: 10.1039/D1TB00935D
PICCIOTTO SABRINA, BARONE MARIA E., FIERLI DAVID, ARANYOS ANITA, ADAMO GIORGIA, BOŽIČ DARJA, ROMANCINO DANIELE P., STANLY CHRISTOP: "Isolation of extracellular vesicles from microalgae: towards the production of sustainable and natural nanocarriers of bioactive compounds", BIOMATERIALS SCIENCE., vol. 9, no. 8, 20 April 2021 (2021-04-20), GB , pages 2917 - 2930, XP055979612, ISSN: 2047-4830, DOI: 10.1039/D0BM01696A
KOH, Yu Ri et al. Skin-regenerative exosome-mimetic nanovesicles fabricated by euglena gracilis microalgae extrusion. 2021 한국공업화학회 추계 연구 논문 초록 (Abstracts Presented at the 63th KSIEC Meeting). 03 November 2021, p. 371, 2P-477.
KANG, Jeong Yi et al. Facile and Large-scale fabrication of exosome-analogous nanovesicles by extrusion of cells with novel cell membrane detergent. 창립 30주년 기념 2020 한국공업화학회 학술대회 연구논문 초록 (Abstracts Presented at 2020 KSIEC Annual Meeting in Celebration of the 30th Anniversary). 2020, pp. 393-394, 3P-486.
CHO JUNG HYEON, KANG JEONG YI, KIM SEULGI, BAEK HWI RA, KIM JUNOH, JANG KWANG-SUK, KIM JIN WOONG: "Skin protein-derived peptide-conjugated vesicular nanocargos for selected skin cell targeting and consequent activation", JOURNAL OF MATERIALS CHEMISTRY. B, vol. 9, no. 24, 23 June 2021 (2021-06-23), GB , pages 4956 - 4962, XP093031174, ISSN: 2050-750X, DOI: 10.1039/D1TB00935D
PICCIOTTO SABRINA, BARONE MARIA E., FIERLI DAVID, ARANYOS ANITA, ADAMO GIORGIA, BOŽIČ DARJA, ROMANCINO DANIELE P., STANLY CHRISTOP: "Isolation of extracellular vesicles from microalgae: towards the production of sustainable and natural nanocarriers of bioactive compounds", BIOMATERIALS SCIENCE., vol. 9, no. 8, 20 April 2021 (2021-04-20), GB , pages 2917 - 2930, XP055979612, ISSN: 2047-4830, DOI: 10.1039/D0BM01696A
KOH, Yu Ri et al. Skin-regenerative exosome-mimetic nanovesicles fabricated by euglena gracilis microalgae extrusion. 2021 한국공업화학회 추계 연구 논문 초록 (Abstracts Presented at the 63th KSIEC Meeting). 03 November 2021, p. 371, 2P-477.
Attorney, Agent or Firm:
NAM, Gun Pil et al. (KR)
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