Title:
MICROARRAY BASED MULTIPLEX PATHOGEN ANALYSIS AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/173695
Kind Code:
A3
Abstract:
Provided herein is a three-dimensional lattice microarray system for DNA sequence detection and analysis and kits including components of the same. In the system a solid support has a plurality of bifunctional polymer linkers, optionally with a fluorescent label, each with a top domain and a bottom end attached thereto at the bottom end and a plurality of nucleic acid probes attached to the top domain of the plurality of bifunctional polymer linkers. Also provided is a method for fabricating the microarray system and methods to detect and identify pathogens in a plant sample or to determine copy number of a pathogen DNA in an unpurified nucleic acid sample by using a known copy number of synthetic DNA as an internal standard.
Inventors:
HOGAN MICHAEL (US)
MAY MELISSA (US)
EGGERS FREDERICK (US)
MAY MELISSA (US)
EGGERS FREDERICK (US)
Application Number:
PCT/US2019/021317
Publication Date:
December 26, 2019
Filing Date:
March 08, 2019
Export Citation:
Assignee:
PATHOGENDX INC (US)
International Classes:
C12Q1/6809; C12Q1/6837; C12Q1/6853; C12Q1/689; C12Q1/6893; C12Q1/6895; G01N33/533; G16B20/10; G16B25/20; G16B25/30
Domestic Patent References:
| WO1989011548A1 | 1989-11-30 | |||
| WO2003043402A2 | 2003-05-30 | |||
| WO1998020019A1 | 1998-05-14 | |||
| WO2016203740A1 | 2016-12-22 |
Foreign References:
| EP1417337B1 | 2009-03-04 | |||
| US20080299578A1 | 2008-12-04 |
Other References:
WU Q. ET AL.: "An Activated GOPS-poly-L-Lysine- Coated Glass Surface for the Immobilization of 60mer Oligonucleotides", ENGINEERING IN LIFE SCIENCES, vol. 5, no. 5, 2005, pages 466 - 470, XP055668929
DEYONG ZHANG ET AL.: "Differentiation of Cucumber mosaic virus isolates by hybridization to oligonucleo-tides in a microarray format", JOURNAL OF VIROLOGICAL METHODS, vol. 123, no. 1, 2005, pages 101 - 108, XP027667294
SHARMA RANJANA ET AL.: "Detection of Transgenic and Endogenous Plant DNA in Digesta and Tissues of Sheep and Pigs Fed Roundup Ready Canola Meal.", J. AGRIC. FOOD CHEM., vol. 54, 2006, pages 1699 - 1709, XP055668933
CHO JAE-CHANG ET AL.: "Quantitative detection of microbial genes by using DNA microarrays", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 68, no. 3, 2002, pages 1425 - 1430, XP055668932
DEYONG ZHANG ET AL.: "Differentiation of Cucumber mosaic virus isolates by hybridization to oligonucleo-tides in a microarray format", JOURNAL OF VIROLOGICAL METHODS, vol. 123, no. 1, 2005, pages 101 - 108, XP027667294
SHARMA RANJANA ET AL.: "Detection of Transgenic and Endogenous Plant DNA in Digesta and Tissues of Sheep and Pigs Fed Roundup Ready Canola Meal.", J. AGRIC. FOOD CHEM., vol. 54, 2006, pages 1699 - 1709, XP055668933
CHO JAE-CHANG ET AL.: "Quantitative detection of microbial genes by using DNA microarrays", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 68, no. 3, 2002, pages 1425 - 1430, XP055668932
Attorney, Agent or Firm:
ADLER, Benjamin, Aaron (US)
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