Title:
MICROARRAY FOR THE DETECTION OF ENTEROPATHOGENIC MICROORGANISMS IN ENVIRONMENTAL AND BIOLOGICAL SAMPLES
Document Type and Number:
WIPO Patent Application WO/2015/152703
Kind Code:
A3
Abstract:
The invention relates to the field of multi-etiological molecular diagnosis and diagnosis of emerging diseases through the use of DNA arrays. More specifically, the invention relates to oligonucleotides included in an array for the detection of microorganisms that can cause diarrheal disease. Said array contains the oligonucleotides corresponding to Enteroaggregative Escherichia coli, Enterohemorrhagic Escherichia coli, Enterotoxigenic Escherichia coli, Enteropathogenic Escherichia coli, Enteroinvasive Escherichia coli, Giardia intestinalis, Blastocystis hominis, Entamoeba histolytica/dispar, Cryptosporidium parvum, Shigella spp., Uropathogenic Escherichia coli, Salmonella enterica, Vibrio cholerae, among other pathogens that cause gastric diseases in humans or animals. The array consists in immobilising said oligonucleotides in a support once they have been amplified using the PCR technique. The array can be a microarray or a macroarray, and, in one embodiment, it takes the form of a biochip, either a macrochip or microchip.
Inventors:
SOUZA SALDIVAR VALERIA FRANCISCA LEOPOLDINA DE MAR (MX)
EGUIARTE FRUNS LUIS ENRIQUE (MX)
GONZÁLEZ GONZÁLEZ ANDREA (US)
DELGADO SAPIEN GABRIELA (MX)
MORALES ESPINOSA MARIA DEL ROSARIO (MX)
SANCHEZ REYES LUNA LUISA (MX)
XIMENEZ GARCIA CECILIA TERESA (MX)
CERRITOS FLORES RENÉ (MX)
ZEMEÑO LEÓN VALERIA (MX)
ALCARAZ PERAZA LUIS DAVID (MX)
EGUIARTE FRUNS LUIS ENRIQUE (MX)
GONZÁLEZ GONZÁLEZ ANDREA (US)
DELGADO SAPIEN GABRIELA (MX)
MORALES ESPINOSA MARIA DEL ROSARIO (MX)
SANCHEZ REYES LUNA LUISA (MX)
XIMENEZ GARCIA CECILIA TERESA (MX)
CERRITOS FLORES RENÉ (MX)
ZEMEÑO LEÓN VALERIA (MX)
ALCARAZ PERAZA LUIS DAVID (MX)
Application Number:
PCT/MX2015/000051
Publication Date:
January 14, 2016
Filing Date:
March 25, 2015
Export Citation:
Assignee:
UNIV NAC AUTÓNOMA DE MÉXICO (MX)
International Classes:
C12Q1/68; C12R1/00; C12R1/19; C12R1/63
Domestic Patent References:
| WO2014001648A1 | 2014-01-03 | |||
| WO2008140494A2 | 2008-11-20 | |||
| WO2002059355A2 | 2002-08-01 |
Foreign References:
| EP2559761A1 | 2013-02-20 |
Other References:
LIU JIE ET AL.: "A laboratory-developed TaqMan Array Card for simultaneous detection of 19 enteropathogens''.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 51, no. 2, 2013, pages 472 - 480, ISSN: 1098-660X
FUKUSHIMA HIROSHI ET AL.: "Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis.", INTERNATIONAL JOURNAL OF MICROBIOLOGY, vol. 2010, 2010, pages 18 pages
POIRIER PHILIPPE ET AL.: "Development and evaluation of a real-time PCR assay for detection and quantification of blastocystis parasites in human stool samples: prospective study of patients with hematological malignancies.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 49, no. 3, 2011, pages 975 - 983, ISSN: 1098-660X
WAWRZYNIAK IVAN ET AL.: "Blastocystis,an unrecognized parasite: an overview of pathogenesis and diagnosis", THERAPEUTIC ADVANCES IN INFECTIOUS DISEASE, vol. 1, no. 5, 2013, pages 167 - 178, ISSN: 2049-9361
FUKUSHIMA HIROSHI ET AL.: "Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis.", INTERNATIONAL JOURNAL OF MICROBIOLOGY, vol. 2010, 2010, pages 18 pages
POIRIER PHILIPPE ET AL.: "Development and evaluation of a real-time PCR assay for detection and quantification of blastocystis parasites in human stool samples: prospective study of patients with hematological malignancies.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 49, no. 3, 2011, pages 975 - 983, ISSN: 1098-660X
WAWRZYNIAK IVAN ET AL.: "Blastocystis,an unrecognized parasite: an overview of pathogenesis and diagnosis", THERAPEUTIC ADVANCES IN INFECTIOUS DISEASE, vol. 1, no. 5, 2013, pages 167 - 178, ISSN: 2049-9361
Attorney, Agent or Firm:
FIGUEROA PÉREZ, Martha (3er Piso Oficinas Administrativas Exteriores,Zona Cultural de Ciudad Universitari, Distrito Federal ., MX)
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