Title:
MICROARRAY QUALITY CONTROL
Document Type and Number:
WIPO Patent Application WO2006058031
Kind Code:
A3
Abstract:
The present invention relates to methods of quality control of manufactured nucleic acid arrays. Fluorescence detection is used to evaluate the quality of a printed nucleic acid array prior to its use in hybridization without the need to add or otherwise link a fluorescent compound or dye to the nucleic acid. Nucleic acid arrays suitable for this method are those where the spots of the array are formed by printing a solution that contains the nucleic acid in an ion containing solution. Printing quality may be evaluated by measuring the intensity of fluorescence at the location of each printed sample, and/or by measuring the "morphology" (i.e. shape) of the printed sample. Printed spots can be "imaged" by measuring fluorescence across a spotted sample in two dimensions. The resulting image of a printed spot can be compared with a reference printed image expected for the printing equipment and solid phase used.
Inventors:
MOHAMMED MANSOOR S (US)
DZIDIC NATASA (US)
DZIDIC NATASA (US)
Application Number:
PCT/US2005/042377
Publication Date:
November 30, 2006
Filing Date:
November 22, 2005
Export Citation:
Assignee:
QUEST DIAGNOSTICS INC (US)
MOHAMMED MANSOOR S (US)
DZIDIC NATASA (US)
MOHAMMED MANSOOR S (US)
DZIDIC NATASA (US)
International Classes:
B41J29/393; B05B1/08; C08F265/10; C12N7/08; C12Q1/68; G01N33/53
Foreign References:
| US20040112980A1 | 2004-06-17 | |||
| US20030013124A1 | 2003-01-16 | |||
| US20020182607A1 | 2002-12-05 | |||
| US5707811A | 1998-01-13 | |||
| US5690894A | 1997-11-25 | |||
| US5405774A | 1995-04-11 |
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