Title:
MICROBIAL CONCENTRATION METHOD USING DIATOMITE AND NUCLEIC ACID EXTRACTION METHOD
Document Type and Number:
WIPO Patent Application WO/2018/169307
Kind Code:
A1
Abstract:
The present invention relates to a microbial concentration method using diatomite and a nucleic acid extraction method. A technique was developed that can achieve approximately 4-8 times the microbial concentration by using diatomite when compared with an existing prior, and a method was developed that can directly extracts nucleic acids from concentrated microorganisms by using dimethyl adipimidate (DMA) or the like. The microbial concentration and nucleic acid extraction techniques allowable in a single tube is expected to significantly reduce contamination coming from the outside, costs, time, complexity, and the like.
Inventors:
SHIN YONG (KR)
ZHAO FEI (KR)
ZHAO FEI (KR)
Application Number:
PCT/KR2018/002994
Publication Date:
September 20, 2018
Filing Date:
March 14, 2018
Export Citation:
Assignee:
UNIV ULSAN FOUND IND COOP (KR)
ASAN FOUND (KR)
ASAN FOUND (KR)
International Classes:
C12Q1/6806; C01B33/12; C12N15/10; G01N1/40
Foreign References:
| US20160215325A1 | 2016-07-28 | |||
| KR20150096444A | 2015-08-24 | |||
| US20100239679A1 | 2010-09-23 | |||
| KR20130106743A | 2013-09-30 |
Other References:
MIGLIACCIO, N.: "Biosilica Nanovector from Diatomite for siRNA Transport in Cancer Cell, thesis", 2015, University of Naples Federico II, Molecular Medicine and Medical Biotechnology, pages 1 - 36, XP055548143
See also references of EP 3597773A4
See also references of EP 3597773A4
Attorney, Agent or Firm:
TAEBAEK INTELLECTUAL PROPERTY LAW FIRM (KR)
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