Title:
MICROBIAL NANOWIRES WITH INCREASED CONDUCTIVITY AND REDUCED DIAMETERS
Document Type and Number:
WIPO Patent Application WO/2017/015306
Kind Code:
A3
Abstract:
Improved electrically conductive pili were generated from a natural pilus protein from the microorganism Geobacter sulfurreducens. Substituting a tryptophan for the phenylalanine at position F51 and a tryptophan for the tyrosine at position Y57 of the pilus monomer substantially increased the conductivity of the pili and reduced their diameter to 1.5 nm. Substantial improvements in conductivity were also achieved by substituting an additional tyrosine, histidine, and phenylalanine in the pilus monomer to mimic the monomer of Geobacter metallireducens, but the pili retained the typical Geobacter sulfurreducens wild-type diameter of 3 nm.
Inventors:
LOVLEY DEREK R (US)
MALVANKAR NIKHIL (US)
ADHIKARI RAMESH (US)
TAN YANG (US)
WARD JOY (US)
NEVIN KELLY (US)
MALVANKAR NIKHIL (US)
ADHIKARI RAMESH (US)
TAN YANG (US)
WARD JOY (US)
NEVIN KELLY (US)
Application Number:
PCT/US2016/043018
Publication Date:
June 08, 2017
Filing Date:
July 19, 2016
Export Citation:
Assignee:
UNIV MASSACHUSETTS (US)
International Classes:
C12N1/21; C12N1/20
Other References:
RICHTER: "Mutational analysis of geopilin function in Geobacter sulfurreducens.", 2011, XP055387745, Retrieved from the Internet [retrieved on 20161228]
REARDON ET AL.: "Structure of the type IVa major pilin from the electrically conductive bacterial nanowires of Geobacter sulfurreducens.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 288, no. 41, 2013, pages 29260 - 29266, XP055387747
REARDON ET AL.: "Structure of the type IVa major pilin from the electrically conductive bacterial nanowires of Geobacter sulfurreducens.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 288, no. 41, 2013, pages 29260 - 29266, XP055387747
Attorney, Agent or Firm:
MILSTEIN, Joseph, B. (US)
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