Title:
MICROBIAL PRODUCTION OF L-ASCORBIC ACID
Document Type and Number:
WIPO Patent Application WO2005017159
Kind Code:
A3
Abstract:
The present invention discloses an isolated polynucleotide molecule derived from a polynucleotide encoding a polypeptide having L-sorbosone dehydrogenase activity comprising a partial nucleotide sequence of at least 20 consecutive nucleotides of SEQ ID NO:1. The present invention further relates to a process for the production of L-ascorbic acid in high yield, in particular a process using resting cells of a microorganism able to convert given carbon sources into vitamin C. The thus obtained vitamin C may be further processed by purification and/or separation steps.
More Like This:
Inventors:
BERRY ALAN (US)
LEE CONNIE (FR)
MAYER ANNE FRANCOISE (CH)
SHINJOH MASAKO (CH)
LEE CONNIE (FR)
MAYER ANNE FRANCOISE (CH)
SHINJOH MASAKO (CH)
Application Number:
PCT/CH2004/000511
Publication Date:
November 03, 2005
Filing Date:
August 16, 2004
Export Citation:
Assignee:
DSM IP ASSETS BV (NL)
BERRY ALAN (US)
LEE CONNIE (FR)
MAYER ANNE FRANCOISE (CH)
SHINJOH MASAKO (CH)
BERRY ALAN (US)
LEE CONNIE (FR)
MAYER ANNE FRANCOISE (CH)
SHINJOH MASAKO (CH)
International Classes:
C12N1/21; C12N9/02; C12N9/04; C12N15/53; C12P7/60; C12P17/04; (IPC1-7): C12N15/53; C12N15/11; C12N9/02; C12N9/04; C12N15/63; C12N1/21; C12P17/04; C12P7/60
Domestic Patent References:
| WO1997004101A2 | 1997-02-06 | |||
| WO2003016508A2 | 2003-02-27 | |||
| WO2003104445A1 | 2003-12-18 | |||
| WO2004029269A1 | 2004-04-08 | |||
| WO2003089634A1 | 2003-10-30 | |||
| WO2004029235A2 | 2004-04-08 |
Foreign References:
| EP0922759A2 | 1999-06-16 | |||
| EP0911415A2 | 1999-04-28 |
Other References:
SAITO Y ET AL: "CLONING OF GENES CODING FOR L-SORBOSE AND L-SORBOSONE DEHYDROGENASES FROM GLUCONOBACTER OXYDANS AND MICROBIAL PRODUCTION OF 2-KETO-L-GULONATE, A PRECURSOR OF L-ASCORBIC ACID, IN A RECOMBINANT G. OXYDANS STRAIN", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 63, no. 2, 1997, pages 454 - 460, XP000886144, ISSN: 0099-2240
DATABASE EMBL [online] 18 December 2001 (2001-12-18), "Agrobacterium tumefaciens str. C58 linear chromosome, section 35 of 187 of the complete sequence.", XP002321379, retrieved from EBI accession no. EM_PRO:AE009265 Database accession no. AE009265
DATABASE UniProt [online] 1 June 2003 (2003-06-01), "Glucose dehydrogenase.", XP002321380, retrieved from EBI accession no. UNIPROT:Q882Q7 Database accession no. Q882Q7
SUGISAWA T ET AL: "ISOLATION AND CHARACTERIZATION OF A NEW VITAMIN C PRODUCING ENZYME (L-GULONO-GAMMA-LACTONE DEHYDROGENASE) OF BACTERIAL ORIGIN", BIOSCIENCE, BIOTECHNOLOGY AND BIOCHEMISTRY, XX, XX, vol. 59, no. 2, February 1995 (1995-02-01), pages 190 - 196, XP001084987, ISSN: 0916-8451
SUGISAWA T ET AL: "MICROBIAL PRODUCTION OF 2-KETO-L-GULONIC ACID FROM L-SORBOSE AND D-SORBITOL BY GLUCONOBACTER MELANOGENUS", AGRICULTURAL AND BIOLOGICAL CHEMISTRY, JAPAN SOC. FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEM. TOKYO, JP, vol. 54, no. 5, May 1990 (1990-05-01), pages 1201 - 1209, XP002061325, ISSN: 0002-1369
LEE H-W ET AL: "Screening for L-sorbose and L-sorbosone dehydrogenase producing microbes for 2-keto-L-gulonic acid production", JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY, BASINGSTOKE, GB, vol. 23, no. 2, August 1999 (1999-08-01), pages 106 - 111, XP002241676, ISSN: 1367-5435
SHINJOH M ET AL: "Cloning and Nucleotide Sequencing of the Membrane-Bound L-Sorbosone Dehydrogenase Gene of Acetobacter liquefaciens IFO 12258, and Its Expression in Gluconobacter oxydans", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 61, no. 2, February 1995 (1995-02-01), pages 413 - 420, XP002267229, ISSN: 0099-2240
DATABASE EMBL [online] 18 December 2001 (2001-12-18), "Agrobacterium tumefaciens str. C58 linear chromosome, section 35 of 187 of the complete sequence.", XP002321379, retrieved from EBI accession no. EM_PRO:AE009265 Database accession no. AE009265
DATABASE UniProt [online] 1 June 2003 (2003-06-01), "Glucose dehydrogenase.", XP002321380, retrieved from EBI accession no. UNIPROT:Q882Q7 Database accession no. Q882Q7
SUGISAWA T ET AL: "ISOLATION AND CHARACTERIZATION OF A NEW VITAMIN C PRODUCING ENZYME (L-GULONO-GAMMA-LACTONE DEHYDROGENASE) OF BACTERIAL ORIGIN", BIOSCIENCE, BIOTECHNOLOGY AND BIOCHEMISTRY, XX, XX, vol. 59, no. 2, February 1995 (1995-02-01), pages 190 - 196, XP001084987, ISSN: 0916-8451
SUGISAWA T ET AL: "MICROBIAL PRODUCTION OF 2-KETO-L-GULONIC ACID FROM L-SORBOSE AND D-SORBITOL BY GLUCONOBACTER MELANOGENUS", AGRICULTURAL AND BIOLOGICAL CHEMISTRY, JAPAN SOC. FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEM. TOKYO, JP, vol. 54, no. 5, May 1990 (1990-05-01), pages 1201 - 1209, XP002061325, ISSN: 0002-1369
LEE H-W ET AL: "Screening for L-sorbose and L-sorbosone dehydrogenase producing microbes for 2-keto-L-gulonic acid production", JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY, BASINGSTOKE, GB, vol. 23, no. 2, August 1999 (1999-08-01), pages 106 - 111, XP002241676, ISSN: 1367-5435
SHINJOH M ET AL: "Cloning and Nucleotide Sequencing of the Membrane-Bound L-Sorbosone Dehydrogenase Gene of Acetobacter liquefaciens IFO 12258, and Its Expression in Gluconobacter oxydans", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 61, no. 2, February 1995 (1995-02-01), pages 413 - 420, XP002267229, ISSN: 0099-2240
Download PDF:
Previous Patent: A NEW CLASS OF &agr 2&dgr CALCIUM CHANNEL SUBUNITS
Next Patent: MOBILIZATION OF HEMATOPOIETIC CELLS
Next Patent: MOBILIZATION OF HEMATOPOIETIC CELLS