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Title:
MICROFLUIDIC CHIP-BASED RAPID IMMUNOASSAY METHOD
Document Type and Number:
WIPO Patent Application WO/2021/169089
Kind Code:
A1
Abstract:
A microfluidic chip-based rapid immunoassay method, comprising the following steps: S1, fabricating a detection chip; S2, immunocapturing a sample to be detected; S3, immunocapturing a probe-modified secondary antibody; and S4, detecting an immunocapture result. Rapid detection is achieved, multiple samples may be jointly detected by means of a one-time operation, and the method has the advantages of small sample size, multiple samples, multiple indicators capable of being detected at the same time, strong specificity, high sensitivity, etc. The method has good sample compatibility, and is applicable to various medium samples to be detected that comprise buffer solutions, serum, plasma, etc. The method does not need complex instruments and devices, and also does not need the empirical determination of test personnel, thus avoiding subjective factors of different operators. The method is especially suitable for on-site detection and immunoreaction kinetics of important respiratory viruses such as the 2019 novel Coronavirus (NCP) and Severe Acute Respiratory Syndrome (SARS) virus.

Inventors:
ZHOU JIANHUA (CN)
LIU YIREN (CN)
Application Number:
PCT/CN2020/096717
Publication Date:
September 02, 2021
Filing Date:
June 18, 2020
Export Citation:
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Assignee:
UNIV SUN YAT SEN (CN)
International Classes:
G01N33/543; G01N33/569
Domestic Patent References:
WO2005064347A12005-07-14
Foreign References:
CN109499636A2019-03-22
CN109557150A2019-04-02
CN107966558A2018-04-27
CN101221167A2008-07-16
CN104931711A2015-09-23
CN103616427A2014-03-05
Attorney, Agent or Firm:
YOGO PATENT AND TRADEMARK AGENCY LIMITED COMPANY (CN)
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