Title:
MICROFLUIDIC CONTROLLING TECHNOLOGY FOR SINGLE-STEP, CONTINUOUS PREPARATION OF CALCIUM ALGINATE MICROGEL
Document Type and Number:
WIPO Patent Application WO/2019/091145
Kind Code:
A1
Abstract:
The invention relates to a single-step, continuous preparation method of a multi-void calcium alginate microgel based on microfluidic droplet technology. The calcium alginate microgel can be used to immobilize bioactive substances. The method can achieve high-throughput and continuous production of a microgel material. The method includes: forming an aqueous solution having a multi-phase parallel flow by injecting a plurality of hydrogel prepolymer solutions into a microfluidic chip channel, in which the hydrogel prepolymer solutions form a parallel, steady flow field in the microfluidic chip channel and constitute a plurality of voids in the subsequently formed multi-void microgel, forming a water-in-oil emulsion droplet by mixing the aqueous solution with an immiscible fluid (oil phase) by means of a T-shaped microfluidic channel or a hydrodynamic focusing microfluidic channel, immediately initiating crosslinking of the calcium alginate in the water-in-oil emulsion droplet to obtain a multi-void microgel; and cleaning the water-in-oil emulsion droplet in the microfluidic chip, such that the multi-void microgel is rapidly transported to the aqueous phase. The method achieves single-step preparation of a calcium alginate microgel capable of immobilizing bioactive substances and is suitable for industrial application.
Inventors:
WANG HUANAN (CN)
ZHANG LIYUAN (CN)
ZHANG LIYUAN (CN)
Application Number:
PCT/CN2018/097726
Publication Date:
May 16, 2019
Filing Date:
July 30, 2018
Export Citation:
Assignee:
SHENZHEN HUA NOVA BIOTECHNOLOGY LTD (CN)
International Classes:
B01J13/00
Foreign References:
| CN107930542A | 2018-04-20 | |||
| CN107298767A | 2017-10-27 | |||
| CN106040117A | 2016-10-26 | |||
| CN104829850A | 2015-08-12 | |||
| CN103656754A | 2014-03-26 | |||
| CN105641743A | 2016-06-08 |
Other References:
YUEBI HU: "Controlled self-assembly of alginate microgels by rapidly binding molecule pairs", LAB ON A CHIP, vol. 17, no. 14, 12 June 2017 (2017-06-12), pages 2481 - 2490, XP055607427, ISSN: 1473-0197
ANGELO S. MAO: "Deterministic encapsulation of single cells in thin tunable microgels for niche modelling and therapeutic delivery", NATURE MATERIALS, 31 October 2016 (2016-10-31), XP055498142, ISSN: 1476-1122
LIYUAN ZHANG: "Microfluidic Templated Multicompartment Microgels for 3D Encapsulation and Pairing of Single Cells", SMALL, vol. 14, no. 9, 15 January 2018 (2018-01-15), pages 1702955 - (1-8), XP055607428, ISSN: 1613-6810
ANGELO S. MAO: "Deterministic encapsulation of single cells in thin tunable microgels for niche modelling and therapeutic delivery", NATURE MATERIALS, 31 October 2016 (2016-10-31), XP055498142, ISSN: 1476-1122
LIYUAN ZHANG: "Microfluidic Templated Multicompartment Microgels for 3D Encapsulation and Pairing of Single Cells", SMALL, vol. 14, no. 9, 15 January 2018 (2018-01-15), pages 1702955 - (1-8), XP055607428, ISSN: 1613-6810
Attorney, Agent or Firm:
DALIAN EAST PATENT AGENT LTD. (CN)
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