Title:
MICROFLUIDIC SYSTEM FOR THE MANIPULATION AND CONCENTRATION OF PARTICLES SUSPENDED IN LIQUID
Document Type and Number:
WIPO Patent Application WO2002092222
Kind Code:
A3
Abstract:
A microfluidic system for the concentration of particulate matter comprises a first reservoir (1) containing a buffer solution, a second reservoir (2) containing a analyte, and a third reservoir (3) containing beads suspended in a liquid. Microchannels link the reservoirs and an expanded portion (4). Hydrostatic pressure is applied to the reservoir (3) containing the beads while an electro-osmotic force (EOF) is applied between the reservoir (1) and the reservoir (3) to establish counter flows of liquids. A vortex forms in the flared portion (5) due to the counter flow of the liquids and the beads are concentrated in the vortex. By switching the EOF between reservoirs (1) and (2) the buffer solution can be replaced by the analyte to enable an analysis to be performed.
Inventors:
BOER GERBEN (CH)
DODGE ARASH (CH)
LETTIERI GIANLUCA (CH)
VERPOORTE ELISABETH (CH)
DODGE ARASH (CH)
LETTIERI GIANLUCA (CH)
VERPOORTE ELISABETH (CH)
Application Number:
PCT/EP2002/005324
Publication Date:
August 28, 2003
Filing Date:
May 13, 2002
Export Citation:
Assignee:
SUISSE ELECTRONIQUE MICROTECH (CH)
BOER GERBEN (CH)
DODGE ARASH (CH)
LETTIERI GIANLUCA (CH)
VERPOORTE ELISABETH (CH)
BOER GERBEN (CH)
DODGE ARASH (CH)
LETTIERI GIANLUCA (CH)
VERPOORTE ELISABETH (CH)
International Classes:
B01L3/00; (IPC1-7): B01L3/00; B01F5/02; B01F13/00; B03C5/00; F15D1/00; G01N15/14
Domestic Patent References:
| WO1990003538A1 | 1990-04-05 | |||
| WO1998046986A1 | 1998-10-22 | |||
| WO2000000293A1 | 2000-01-06 |
Foreign References:
| US6170981B1 | 2001-01-09 | |||
| US6074827A | 2000-06-13 |
Other References:
OLESCHUK R D ET AL: "TRAPPING OF BEAD-BASED REAGENTS WITHIN MICROFLUIDIC SYNSTEMS: ON-CHIP SOLID-PHASE EXTRACTION AND ELECTROCHROMATOGRAPHY", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 3, no. 72, 1 February 2000 (2000-02-01), pages 585 - 590, XP001074756, ISSN: 0003-2700
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