Title:
MICRONEEDLE PATCH FOR ENHANCING ACCUMULATION OF PROTOPORPHYRIN IX IN SOLID TUMOR AND PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2023/178878
Kind Code:
A1
Abstract:
A microneedle patch for enhancing an accumulation of protoporphyrin IX in a solid tumor and a preparation method therefor. The method comprises the steps of: dissolving albumin in a Dulbecco's culture medium, using the albumin as a template, and adding CuCl2 and CaCl2 for mineralization to obtain a CCP nanoparticle; by means of a one-step carbodiimide coupling method and an electrostatic adsorption method, loading CAT and 5-ALA to the surface of the CCP nanoparticle, respectively, to obtain a CCPCA nanoparticle; and loading the CCPCA nanoparticle and a microneedle matrix solution in a microneedle mold, drying under vacuum, and demolding to obtain a microneedle patch for enhancing an accumulation of PpIX in a solid tumor. According to the microneedle patch, by means of an integrated nano calcium phosphate carrier and a transdermal administration mode, an accurate delivery of CAT and 5-ALA is achieved, and PpIX and O2 levels in a tumor cell are increased. Meanwhile, the guidance of real-time bimodal PpIX fluorescence imaging and photoacoustic blood oxygen imaging is provided, and the photodynamic therapeutic effect on different cancers is improved.
Inventors:
HUANG PENG (CN)
HE GANG (CN)
LI YASHI (CN)
LIN JING (CN)
HE GANG (CN)
LI YASHI (CN)
LIN JING (CN)
Application Number:
PCT/CN2022/103330
Publication Date:
September 28, 2023
Filing Date:
July 01, 2022
Export Citation:
Assignee:
UNIV SHENZHEN (CN)
International Classes:
A61K41/00; A61K9/70; A61K47/42; A61K49/00; A61K49/22; A61M37/00; A61P35/00
Foreign References:
| CN111297830A | 2020-06-19 |
Other References:
FU LIAN‐HUA, WAN YILIN, LI CHUNYING, QI CHAO, HE TING, YANG CHEN, ZHANG YIFAN, LIN JING, HUANG PENG: "Biodegradable Calcium Phosphate Nanotheranostics with Tumor‐Specific Activatable Cascade Catalytic Reactions‐Augmented Photodynamic Therapy", ADVANCED FUNCTIONAL MATERIALS, WILEY - V C H VERLAG GMBH & CO. KGAA, DE, vol. 31, no. 14, 1 April 2021 (2021-04-01), DE , XP093095868, ISSN: 1616-301X, DOI: 10.1002/adfm.202009848
HU XIULI, ZHANG HUIJUAN, WANG ZEJUN, SHIU CHIN YING ANGELA, GU ZHEN: "Microneedle Array Patches Integrated with Nanoparticles for Therapy and Diagnosis", SMALL STRUCTURES, WILEY, vol. 2, no. 4, 1 April 2021 (2021-04-01), XP093095970, ISSN: 2688-4062, DOI: 10.1002/sstr.202000097
赵笑等 (ZHAO, XIAO ET AL.): "负载5-氨基乙酰丙酸透明质酸微针的制备及其光动力治疗 (Non-official translation: Preparation and Photodynamic Treatment of 5-Aminolevulinic Acid Hyaluronic Acid Microneedles)", 中国化学会2017全国高分子学术论文报告会摘要集-主题F: 生物医用高分子 (NON-OFFICIAL TRANSLATION: CHINESE CHEMICAL SOCIETY 2017 NATIONAL POLYMER ACADEMIC PAPERS REPORT ABSTRACT COLLECTION-SUBJECT F: BIOMEDICAL POLYMER), 10 October 2017 (2017-10-10), pages 325 - 326, XP009549117
WANG MIN, ZHANG MIAO, FU LIANHUA, LIN JING, ZHOU XINMIN, ZHOU PINGHONG, HUANG PENG, HU HAO, HAN YING: "Liver-targeted delivery of TSG-6 by calcium phosphate nanoparticles for the management of liver fibrosis", THERANOSTICS, IVYSPRING INTERNATIONAL PUBLISHER, AU, vol. 10, no. 1, 1 January 2020 (2020-01-01), AU , pages 36 - 49, XP093095973, ISSN: 1838-7640, DOI: 10.7150/thno.37301
CHEN, WEI ET AL.: "Microneedle-array patches loaded with dual mineralized protein/peptide particles for type 2 diabetes therapy", NATURE COMMUNICATIONS, vol. 8, 24 November 2017 (2017-11-24), pages 1 - 11, XP055710786, DOI: 10.1038/s41467-017-01764-1
HU XIULI, ZHANG HUIJUAN, WANG ZEJUN, SHIU CHIN YING ANGELA, GU ZHEN: "Microneedle Array Patches Integrated with Nanoparticles for Therapy and Diagnosis", SMALL STRUCTURES, WILEY, vol. 2, no. 4, 1 April 2021 (2021-04-01), XP093095970, ISSN: 2688-4062, DOI: 10.1002/sstr.202000097
赵笑等 (ZHAO, XIAO ET AL.): "负载5-氨基乙酰丙酸透明质酸微针的制备及其光动力治疗 (Non-official translation: Preparation and Photodynamic Treatment of 5-Aminolevulinic Acid Hyaluronic Acid Microneedles)", 中国化学会2017全国高分子学术论文报告会摘要集-主题F: 生物医用高分子 (NON-OFFICIAL TRANSLATION: CHINESE CHEMICAL SOCIETY 2017 NATIONAL POLYMER ACADEMIC PAPERS REPORT ABSTRACT COLLECTION-SUBJECT F: BIOMEDICAL POLYMER), 10 October 2017 (2017-10-10), pages 325 - 326, XP009549117
WANG MIN, ZHANG MIAO, FU LIANHUA, LIN JING, ZHOU XINMIN, ZHOU PINGHONG, HUANG PENG, HU HAO, HAN YING: "Liver-targeted delivery of TSG-6 by calcium phosphate nanoparticles for the management of liver fibrosis", THERANOSTICS, IVYSPRING INTERNATIONAL PUBLISHER, AU, vol. 10, no. 1, 1 January 2020 (2020-01-01), AU , pages 36 - 49, XP093095973, ISSN: 1838-7640, DOI: 10.7150/thno.37301
CHEN, WEI ET AL.: "Microneedle-array patches loaded with dual mineralized protein/peptide particles for type 2 diabetes therapy", NATURE COMMUNICATIONS, vol. 8, 24 November 2017 (2017-11-24), pages 1 - 11, XP055710786, DOI: 10.1038/s41467-017-01764-1
Attorney, Agent or Firm:
JOHNSON INTELLECTUAL PROPERTY AGENCY(SHENZHEN) (CN)
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