Title:
MICROORGANISM HAVING IMPROVED GENE PARTICIPATING IN HYDROGEN PRODUCTION ABILITY AND METHOD OF PRODUCING HYDROGEN BY USING THE MICROORGANISM
Document Type and Number:
WIPO Patent Application WO/2006/062130
Kind Code:
A1
Abstract:
It is intended to provide a microorganism having a formate dehydrogenase gene and a hydrogenase gene and further containing a foreign gene of a transcriptional activator in the formate hydrogenlyase system, characterized in that the transcriptional activator in the formate hydrogenlyase system is overexpressed and thus the function of producing hydrogen from formic acid is enhanced; and a method of producing hydrogen by using this microorganism. Use of the above-described microorganism makes it possible to produce hydrogen from an organic substrate at a practically available level. The hydrogen obtained by this technique is free from carbon monoxide causing deterioration in electrode catalysts in fuel batteries and, therefore, is useful as a fuel for fuel batteries.
More Like This:
Inventors:
YUKAWA HIDEAKI (JP)
INUI MASAYUKI (JP)
YOSHIDA AKIHITO (JP)
TORATA NAOTO (JP)
INUI MASAYUKI (JP)
YOSHIDA AKIHITO (JP)
TORATA NAOTO (JP)
Application Number:
PCT/JP2005/022463
Publication Date:
June 15, 2006
Filing Date:
December 07, 2005
Export Citation:
Assignee:
RES INST INNOVATIVE TECH EARTH (JP)
SHARP KK (JP)
YUKAWA HIDEAKI (JP)
INUI MASAYUKI (JP)
YOSHIDA AKIHITO (JP)
TORATA NAOTO (JP)
SHARP KK (JP)
YUKAWA HIDEAKI (JP)
INUI MASAYUKI (JP)
YOSHIDA AKIHITO (JP)
TORATA NAOTO (JP)
International Classes:
C12N15/00; C12N1/21; C12N15/09; C12P3/00; C12R1/19
Domestic Patent References:
| WO2004074495A1 | 2004-09-02 |
Foreign References:
| JP2005087035A | 2005-04-07 |
Other References:
YOSHIDA A. ET AL: "Bio Suiso Seisan: Komitsudo Kintai Hanno Oyobi Idenshi Kairyo ni yoru Ko Seisansei eno Kokoromi", NIPPON NOGEI KAGAKUKAI TAIKAI KOEN YOSHISHU, 2003, pages 38 - LECTURE NO 2A11P09, XP002999328
UNDEN G. ET AL: "Oxygen regulated gene expression in facultatively anaerobic bacteria", ANTONIE VAN LEEUWENHOEK, vol. 66, no. 1-3, 1994, pages 3 - 22, XP002999329
SAUTER M. ET AL: "Mutational analysis of the operon (hyc) determining hydrogenase 3 formation in Escherichia coli", MOL. MICROBIOL., vol. 6, no. 11, 1992, pages 1523 - 1532, XP002999330
SELF W.T. ET AL: "Expression and regulation of a silent operon, hyf, coding for hydrogenase 4 isoenzyme in Escherichia coli", J. BACTERIOL., vol. 186, no. 2, January 2004 (2004-01-01), pages 580 - 587, XP002999331
AXLEY M.J. ET AL: "Escherichia coli formate-hydrogen lyase. Purification and properties of the selenium-dependent formate dehydrogenase component", J. BIOL. CHEM., vol. 265, no. 30, 1990, pages 18213 - 18218, XP002999332
UNDEN G. ET AL: "Oxygen regulated gene expression in facultatively anaerobic bacteria", ANTONIE VAN LEEUWENHOEK, vol. 66, no. 1-3, 1994, pages 3 - 22, XP002999329
SAUTER M. ET AL: "Mutational analysis of the operon (hyc) determining hydrogenase 3 formation in Escherichia coli", MOL. MICROBIOL., vol. 6, no. 11, 1992, pages 1523 - 1532, XP002999330
SELF W.T. ET AL: "Expression and regulation of a silent operon, hyf, coding for hydrogenase 4 isoenzyme in Escherichia coli", J. BACTERIOL., vol. 186, no. 2, January 2004 (2004-01-01), pages 580 - 587, XP002999331
AXLEY M.J. ET AL: "Escherichia coli formate-hydrogen lyase. Purification and properties of the selenium-dependent formate dehydrogenase component", J. BIOL. CHEM., vol. 265, no. 30, 1990, pages 18213 - 18218, XP002999332
Attorney, Agent or Firm:
Iwatani, Ryo (1-31 Dojima 2-chome, Kita-k, Osaka-shi Osaka 03, JP)
Download PDF: