Title:
MICROORGANISM HAVING MULTIPLE GENES ENCODING PHA SYNTHASE AND METHOD FOR PRODUCING PHA USING SAME
Document Type and Number:
WIPO Patent Application WO/2015/146195
Kind Code:
A1
Abstract:
The purpose of the present invention is to improve the crystallization speed of slowly crystallizing PHA copolymer, improve melt workability of PHA copolymer in working by injection molding, film molding, blow molding, fiber spinning, extrusion foaming and bead foaming, and improve productivity. Provided is a method for producing a PHA mixture, the method comprising a step for simultaneously producing in the cells of a microorganism two or more types of PHA having melting points that differ by at least 10˚C, and doing so by culturing the microorganism which has both a gene that encodes PHA synthase derived from the genus Aeromonas that synthesizes copolymer PHA (A) and a gene that encodes PHA synthase that synthesizes PHA (B) having a melting point that differs from that of copolymer PHA (A) by at least 10˚C.
Inventors:
KOBAYASHI SHINGO
AOKI RINA
MINAMI TETSUYA
ARIKAWA HISASHI
MATSUMOTO KEIJI
AOKI RINA
MINAMI TETSUYA
ARIKAWA HISASHI
MATSUMOTO KEIJI
Application Number:
PCT/JP2015/001795
Publication Date:
October 01, 2015
Filing Date:
March 27, 2015
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C12P7/62; C08G63/06; C12N1/21; C08L101/16; C12N15/09
Domestic Patent References:
| WO2009145164A1 | 2009-12-03 | |||
| WO2011105379A1 | 2011-09-01 |
Foreign References:
| JP2004250629A | 2004-09-09 |
Other References:
SUN J. ET AL.: "Production of P(3- hydroxybutyrate-co-3-hydroxyhexanoate-co-3- hydroxyoctanoate) Terpolymers Using a Chimeric PHA Synthase in Recombinant Ralstonia eutropha and Pseudomonas putida", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 74, no. 8, 2010, pages 1716 - 1718, XP055358664
MATSUMOTO K. ET AL.: "Chimeric Enzyme Composed of Polyhydroxyalkanoate (PHA) Synthases from Ralstonia eutropha and Aeromonas caviae Enhances Production of PHAs in Recombinant Escherichia coli", BIOMACROMOLECULES, vol. 10, no. 4, 2009, pages 682 - 685, XP002722989
DATABASE GenBank 18 July 2013 (2013-07-18), "poly-beta-hydroxybutyrate polymerase [Cupriavidus necator]", XP055225668, retrieved from ncbi Database accession no. WP_011615085
See also references of EP 3124615A4
MATSUMOTO K. ET AL.: "Chimeric Enzyme Composed of Polyhydroxyalkanoate (PHA) Synthases from Ralstonia eutropha and Aeromonas caviae Enhances Production of PHAs in Recombinant Escherichia coli", BIOMACROMOLECULES, vol. 10, no. 4, 2009, pages 682 - 685, XP002722989
DATABASE GenBank 18 July 2013 (2013-07-18), "poly-beta-hydroxybutyrate polymerase [Cupriavidus necator]", XP055225668, retrieved from ncbi Database accession no. WP_011615085
See also references of EP 3124615A4
Attorney, Agent or Firm:
PATENT CORPORATE BODY ARCO PATENT OFFICE (JP)
Patent business corporation Owner old patent firm (JP)
Patent business corporation Owner old patent firm (JP)
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