Title:
MICROORGANISM HAVING PHA SYNTHASE-CODING GENES AND METHOD FOR PRODUCING PHA USING SAME
Document Type and Number:
WIPO Patent Application WO/2017/056442
Kind Code:
A1
Abstract:
The purpose of the present invention is to increase the crystallization speed of a PHA copolymer that crystallizes slowly and improve the melt processability thereof to thereby increase productivity. For this purpose, a microorganism that has two or more kinds of different PHA synthase-coding genes originated from the genus Aeromonas is used. It is preferred that the PHA synthase-coding genes originated from the genus Aeromonas include at least two PHA synthase-coding genes that are capable of synthesizing a copolymerized PHA containing 3- hydroxybutyric acid and 3-hydroxyhexanoic acid as monomer units and different from each other in substrate specificity for 3-hydroxyhexanoic acid. By culturing this microorganism, a PHA mixture comprising three or more kinds of PHA's, said PHA's having different melting points, can be produced.
Inventors:
KOBAYASHI SHINGO
SASHIWA HITOSHI
FUJIKI TETSUYA
SASHIWA HITOSHI
FUJIKI TETSUYA
Application Number:
PCT/JP2016/004222
Publication Date:
April 06, 2017
Filing Date:
September 15, 2016
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C12N1/21; C08G63/06; C12N15/09; C12P7/62
Domestic Patent References:
| WO2015133468A1 | 2015-09-11 | |||
| WO2012133231A1 | 2012-10-04 | |||
| WO2015146195A1 | 2015-10-01 |
Foreign References:
| JP2015029484A | 2015-02-16 |
Other References:
LU X. ET AL.: "Molecular cloning and functional analysis of two polyhydroxyalkanoate synthases from two strains of Aeromonas hydrophila spp", FEMS MICROBIOL.LETT., vol. 243, no. 1, 2005, pages 149 - 155, XP027871607
TSUGE T. ET AL.: "Combination of N149S and D171G mutations in Aeromonas caviae polyhydroxyalkanoate synthase and impact on polyhydroxyalkanoate biosynthesis", FEMS MICROBIOL.LETT., vol. 277, no. 2, 2007, pages 217 - 222, XP002702996
TSUGE T. ET AL.: "Variation in copolymer composition and molecular weight of polyhydroxyalkanoate generated by saturation mutagenesis of Aeromonas caviae PHA synthase", MACROMOL.BIOSCI., vol. 7, no. 6, 2007, pages 846 - 854, XP055520079
See also references of EP 3358002A4
TSUGE T. ET AL.: "Combination of N149S and D171G mutations in Aeromonas caviae polyhydroxyalkanoate synthase and impact on polyhydroxyalkanoate biosynthesis", FEMS MICROBIOL.LETT., vol. 277, no. 2, 2007, pages 217 - 222, XP002702996
TSUGE T. ET AL.: "Variation in copolymer composition and molecular weight of polyhydroxyalkanoate generated by saturation mutagenesis of Aeromonas caviae PHA synthase", MACROMOL.BIOSCI., vol. 7, no. 6, 2007, pages 846 - 854, XP055520079
See also references of EP 3358002A4
Attorney, Agent or Firm:
PATENT CORPORATE BODY ARCO PATENT OFFICE (JP)
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