Title:
MICROORGANISMS AND METHODS FOR THE FERMENTATION OF CANNABINOIDS
Document Type and Number:
WIPO Patent Application WO/2020/208411
Kind Code:
A3
Abstract:
Disclosed herein are microorganism and methods that can be used for the synthesis of cannabigerolic acid (CBGA) and cannabinoids. The methods disclosed can be used to produce CBGA, Δ9-tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA), cannabichromenic acid (CBGA), Δ9-tetrahydrocannabivarinic acid (THCVA), cannabidivarinic acid (CBDVA), cannabichromevarinic acid (CBCVA), Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD), cannabichromene (CBC). Enzymes useful for the synthesis of CBGA and cannabinoids, include but are not limited to acyl activating enzyme (AAE1), polyketide synthase (PKS), olivetolic acid cyclase (OAC), prenyltransferase (PT), THCA synthase (THCAS), CBDA synthase (CBDAS), CBC A synthase (CBCAS), HMG-Co reductase (HMG1), and/or famesyl pyrophosphate synthetase (ERG20). The microorganisms can also have one or more genes disrupted, such as gene that that controls beta oxidation of long chain fatty acids.
Inventors:
SZAMECZ BELA (HU)
VARSZEGI SZILVIA (HU)
NEMETH ATTILA (HU)
SZABO LORAND (HU)
VARSZEGI SZILVIA (HU)
NEMETH ATTILA (HU)
SZABO LORAND (HU)
Application Number:
PCT/IB2020/000241
Publication Date:
December 24, 2020
Filing Date:
April 11, 2020
Export Citation:
Assignee:
ELESZTO GENETIKA INC (HU)
International Classes:
C12P7/22; C12N1/18; C12N9/02; C12N9/10; C12N15/52; C12P7/42; C12P15/00; C12P17/06
Domestic Patent References:
| WO2018200888A1 | 2018-11-01 | |||
| WO2019014490A1 | 2019-01-17 | |||
| WO2016010827A1 | 2016-01-21 | |||
| WO2019071000A1 | 2019-04-11 |
Other References:
ZIRPEL B. ET AL: "Engineering yeasts as platform organisms for cannabinoid biosynthesis", JOURNAL OF BIOTECHNOLOGY, vol. 259, 8 July 2017 (2017-07-08), pages 204 - 212, XP085167531, ISSN: 0168-1656, DOI: 10.1016/J.JBIOTEC.2017.07.008
LUO X. ET AL: "Complete biosynthesis of cannabinoids and their unnatural analogues in yeast", NATURE, vol. 567, no. 7746, 27 February 2019 (2019-02-27), pages 123 - 126, XP037063929, ISSN: 0028-0836, [retrieved on 20190227], DOI: 10.1038/S41586-019-0978-9
GONÇALVES J. ET AL: "Cannabis and Its Secondary Metabolites: Their Use as Therapeutic Drugs, Toxicological Aspects, and Analytical Determination", MEDICINES, vol. 6, no. 1, 23 February 2019 (2019-02-23), pages 31, XP055732614, DOI: 10.3390/medicines6010031
LUO X. ET AL: "Complete biosynthesis of cannabinoids and their unnatural analogues in yeast", NATURE, vol. 567, no. 7746, 27 February 2019 (2019-02-27), pages 123 - 126, XP037063929, ISSN: 0028-0836, [retrieved on 20190227], DOI: 10.1038/S41586-019-0978-9
GONÇALVES J. ET AL: "Cannabis and Its Secondary Metabolites: Their Use as Therapeutic Drugs, Toxicological Aspects, and Analytical Determination", MEDICINES, vol. 6, no. 1, 23 February 2019 (2019-02-23), pages 31, XP055732614, DOI: 10.3390/medicines6010031
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