Title:
MICROORGANISMS FOR PRODUCING ISOBUTANOL AND METHODS RELATED THERETO
Document Type and Number:
WIPO Patent Application WO/2012/177601
Kind Code:
A3
Abstract:
Provided herein is a non-naturally occurring microbial organism having an isopropanol pathway and comprising at least one exogenous nucleic acid encoding an isopropanol pathway enzyme. In certain embodiments, the pathway includes an enzyme selected from a 4-hydroxybutyryl-CoA dehydratase, a crotonase, a 3-hydroxybutyryl-CoA dehydrogenase, an acetoacetyl-CoA synthetase, an acetyl-CoA:acetoacetate-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetate decarboxylase, and an acetone reductase. Also provided herein is anon-naturally occurring microbial organism having an n-butanol pathway and comprising at least one exogenous nucleic acid encoding an n-butanol pathway enzyme. Other non-naturally occurring microbial organism having n-butanol or isobutanol pathways are also provided herein. In certain embodiments, isobutanol pathways utilizing reverse TCA and/or reducing equivalents from CO and/or hydrogen are used to enhance product yields. The organisms provided herein can be cultured to produce isopropanol, n-butanol, or isobutanol.
Inventors:
BURGARD ANTHONY P (US)
OSTERHOUT ROBIN E (US)
SUN JUN (US)
PHARKYA PRITI (US)
OSTERHOUT ROBIN E (US)
SUN JUN (US)
PHARKYA PRITI (US)
Application Number:
PCT/US2012/043091
Publication Date:
May 08, 2014
Filing Date:
June 19, 2012
Export Citation:
Assignee:
GENOMATICA INC (US)
BURGARD ANTHONY P (US)
OSTERHOUT ROBIN E (US)
SUN JUN (US)
PHARKYA PRITI (US)
BURGARD ANTHONY P (US)
OSTERHOUT ROBIN E (US)
SUN JUN (US)
PHARKYA PRITI (US)
International Classes:
C07C29/00; C12N1/00; C12N1/20; C12P7/16
Foreign References:
| US20100304453A1 | 2010-12-02 | |||
| US20110129899A1 | 2011-06-02 | |||
| US20100323418A1 | 2010-12-23 |
Attorney, Agent or Firm:
GAY, David, A. et al. (222 East 41st StreetNew York, NY, US)
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