MICROSCOPIC SUBSTANCE ENCAPSULATION METHOD, MICROSCOPIC SUBSTANCE DETECTION METHOD, AND DEVICE FOR DETECTING MICROSCOPIC SUBSTANCE

WIPO Patent Application WO/2018/181443

A1

The present invention provides, as a technology for encapsulating, in a tiny volume of liquid droplet, a to-be-detected substance such as nucleic acid, protein, virus, or cell by means of a simple operation so as to enable highly sensitive detection, a method for encapsulating a microscopic substance in at least some of a plurality of recesses formed in the surface of a substrate so as to be spaced away from each other, the method comprising (1) a first step for disposing an insertion body above the recess-formed surface of the substrate, positioning the relative positions of said insertion body and the substrate by a supporting part provided to the insertion body in such a manner as to have the undersurface of the insertion body and the recess-formed surface of the substrate face each other, thereby providing a solution introduction space between the undersurface of the insertion body and the recess-formed surface of the substrate, and providing a solution discharge space that is connected with the solution introduction space and is provided above the undersurface of the insertion body and between the substrate and the insertion body so as to be positioned within the substrate and/or within the insertion body.

NOJI HIROYUKI (JP)
TABATA KAZUHITO (JP)

PCT/JP2018/012678

October 04, 2018

March 28, 2018

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JAPAN SCIENCE & TECH AGENCY (JP)

G01N1/04; G01N1/00

WO2016006208A1	2016-01-14
WO2012121310A1	2012-09-13
WO2012121310A1	2012-09-13

JP2014503831A	2014-02-13
US20050220675A1	2005-10-06
JP2004309405A	2004-11-04

See also references of EP 3605054A4

OHNO Seiji et al. (JP)

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