Title:
MIDDLE EAR MUCOUS MEMBRANE-LIKE CELL SHEET, PRODUCTION METHOD THEREFOR AND UTILIZATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2012/036246
Kind Code:
A1
Abstract:
To obtain an alternative to a middle-ear-cavity mucous membrane, which engrafts on entotympanic-bone surface, and suppresses the proliferation of granulation tissue and hyperostosis in a middle ear cavity, development of fibroblast, and the like. Nasal epithelial cells and the like are cultured on the cell culture support coated with the polymer in which the hydration force varies in the temperature range of 0 to 80°C on the surface thereof, and middle ear mucous membrane-like cells are cultured in the temperature range in which the hydration force of polymer is weak, thereafter a middle ear mucous membrane-like cell sheet retaining cilia on the surface layer is obtained by collecting a culture fluid in sheet form by varying the temperature of the culture fluid to a temperature at which the hydration force of polymer becomes strong.
Inventors:
HAMA TAKANORI (JP)
KOJIMA HIROMI (JP)
MORIYAMA HIROSHI (JP)
YAMATO MASAYUKI (JP)
OKANO TERUO (JP)
KOJIMA HIROMI (JP)
MORIYAMA HIROSHI (JP)
YAMATO MASAYUKI (JP)
OKANO TERUO (JP)
Application Number:
PCT/JP2011/071147
Publication Date:
March 22, 2012
Filing Date:
September 15, 2011
Export Citation:
Assignee:
UNIV TOKYO WOMENS MEDICAL (JP)
JIKEI UNIVERSITY (JP)
HAMA TAKANORI (JP)
KOJIMA HIROMI (JP)
MORIYAMA HIROSHI (JP)
YAMATO MASAYUKI (JP)
OKANO TERUO (JP)
JIKEI UNIVERSITY (JP)
HAMA TAKANORI (JP)
KOJIMA HIROMI (JP)
MORIYAMA HIROSHI (JP)
YAMATO MASAYUKI (JP)
OKANO TERUO (JP)
International Classes:
A61L27/00; A61F2/18; C12N5/071
Foreign References:
| JP2000125863A | 2000-05-09 | |||
| JP2005130838A | 2005-05-26 | |||
| JP2007222045A | 2007-09-06 | |||
| JP2010226055A | 2010-09-15 | |||
| JPH02211865A | 1990-08-23 | |||
| JPH05192138A | 1993-08-03 | |||
| JP2007080990A | 2007-03-29 |
Other References:
KAZUO YAMAMOTO ET AL.: "Hana Nenmaku Johi Saibo Sheet Ishoku ni yoru Chuji Nenmaku Saisei -Kato o Mochiita Ishoku Jikken", OTOL JPN, vol. 19, no. 4, 16 September 2009 (2009-09-16), pages 623, XP008169925
DAI NAGASE ET AL.: "Histopathological Study of Transplanted Nasal Mucosa in Cases of Operative Ear with Adhesive Otitis Media", JOURNAL OF THE SHOWA MEDICAL ASSOCIATION, vol. 63, no. 2, 28 April 2003 (2003-04-28), pages 211 - 221, XP008169322
MOTOTOSHI KIMURA: "Transplantation of the Mucosa of the Inferior Turbinate for Adhesive Otitis Media. Histomorphological Observation of Transplanted Mucosa of the Inferior Turbinate", JIBIINKOKA TENBO, vol. 34, no. 3, June 1991 (1991-06-01), pages 289 - 298, XP055078681
YUICHIRO TANIGUCHI ET AL.: "Ondo Otosei Baiyozara o Mochiite Sakusei shita Saibo Sheet ni yoru Chuji Nenmaku Saisei", OTOL JPN, vol. 16, no. 4, 22 September 2006 (2006-09-22), pages 297, XP008169926
ANN. OTOL. RHINOL. LARYNGOL., vol. 104, 1995, pages 369 - 373
LARYNGOSCOPE, vol. 108, 1998, pages 1840 - 1845
ACTA. OTO-LARYNGOLOGICA., vol. 127, 2007, pages 1038 - 1044
ACTA. OTO-LARYNGOLOGICA., vol. 126, 2006, pages 801 - 810
See also references of EP 2617438A4
DAI NAGASE ET AL.: "Histopathological Study of Transplanted Nasal Mucosa in Cases of Operative Ear with Adhesive Otitis Media", JOURNAL OF THE SHOWA MEDICAL ASSOCIATION, vol. 63, no. 2, 28 April 2003 (2003-04-28), pages 211 - 221, XP008169322
MOTOTOSHI KIMURA: "Transplantation of the Mucosa of the Inferior Turbinate for Adhesive Otitis Media. Histomorphological Observation of Transplanted Mucosa of the Inferior Turbinate", JIBIINKOKA TENBO, vol. 34, no. 3, June 1991 (1991-06-01), pages 289 - 298, XP055078681
YUICHIRO TANIGUCHI ET AL.: "Ondo Otosei Baiyozara o Mochiite Sakusei shita Saibo Sheet ni yoru Chuji Nenmaku Saisei", OTOL JPN, vol. 16, no. 4, 22 September 2006 (2006-09-22), pages 297, XP008169926
ANN. OTOL. RHINOL. LARYNGOL., vol. 104, 1995, pages 369 - 373
LARYNGOSCOPE, vol. 108, 1998, pages 1840 - 1845
ACTA. OTO-LARYNGOLOGICA., vol. 127, 2007, pages 1038 - 1044
ACTA. OTO-LARYNGOLOGICA., vol. 126, 2006, pages 801 - 810
See also references of EP 2617438A4
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
Aoki 篤 (JP)
Aoki 篤 (JP)
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