Title:
MIR-210 MIMIC, AND PREPARATION METHOD THEREFOR AND USE THEREOF AS ANTIBIOTIC SUBSTITUTE FOR CULTURE
Document Type and Number:
WIPO Patent Application WO/2022/241922
Kind Code:
A1
Abstract:
A miR-210 mimic, wherein the RNA sequence of the miR-210 mimic is 5'-U SU SGUGCGUGCGACAGCGAC SU SG SA S-Chol-3'. The preparation method is carried out in sequence according to the following steps: using an RNA sequence of adult miR-210 to synthesize an oligonucleotide single strand, wherein the RNA sequence of the adult miR-210 is as shown in SEQ ID NO: 1; performing a cholesterol modification on the 3' end of the oligonucleotide single strand; performing two thio-backbone modifications on the 5' end of the oligonucleotide single strand, performing four thio-backbone modifications on the 3' end of the oligonucleotide single strand, and performing a methoxyl modification on the whole oligonucleotide single strand; and purifying the product to obtain the miR-210 mimic having the RNA sequence as shown in SEQ ID NO: 1. The miR-210 mimic can be used as a drug for inhibiting pathogens of skin ulceration syndrome in a Apostichopus japonicus culture or as a drug for inhibiting pathogens of red spotting disease in a sea urchin culture.
Inventors:
ZHAN YAOYAO (CN)
CHANG YAQING (CN)
LIU LI (CN)
ZHAO TANJUN (CN)
SONG JIAN (CN)
CHANG YAQING (CN)
LIU LI (CN)
ZHAO TANJUN (CN)
SONG JIAN (CN)
Application Number:
PCT/CN2021/104504
Publication Date:
November 24, 2022
Filing Date:
July 05, 2021
Export Citation:
Assignee:
UNIV DALIAN OCEAN (CN)
International Classes:
C12N15/113; A23K20/153; A23K50/80; A61K31/7088; A61P31/04
Foreign References:
| CN110484537A | 2019-11-22 |
Other References:
LI CHENGHUA; ZHAO MENGRU; ZHANG CHI; ZHANG WEIWEI; ZHAO XUELIN; DUAN XUEMEI; XU WEI: "miR210 modulates respiratory burst inApostichopus japonicuscoelomocytes via targeting Toll-like receptor", DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, PERGAMON PRESS., US, vol. 65, 18 August 2016 (2016-08-18), US , pages 377 - 381, XP029728000, ISSN: 0145-305X, DOI: 10.1016/j.dci.2016.08.008
ZHANG, YI ET AL.: "MiR-210 Regulates Coelomocyte Proliferation through Targeting E2F3 in Apostichopus Japonicus", FISH AND SHELLFISH IMMUNOLOGY, vol. 106, 21 August 2020 (2020-08-21), XP086300604, DOI: 10.1016/j.fsi.2020.08.036
ZHAN, YAOYAO ET AL.: "MicroRNAs Involved in Innate Immunity Regulation in the Sea Cucumber: A Review", FISH AND SHELLFISH IMMUNOLOGY, vol. 95, 25 October 2019 (2019-10-25), XP085933737, DOI: 10.1016/j.fsi.2019.10.049
ZHAN YAOYAO, LI YINGYING, CUI DONGYAO, PEI QIANTONG, SUN JINGXIAN, ZHANG WEIJIE, CHANG YAQING: "Identification and characterization of microRNAs from the tube foot in the sea urchin Strongylocentrotus intermedius", HELIYON, ELSEVIER LTD, GB, vol. 4, no. 6, 1 June 2018 (2018-06-01), GB , pages e00668, XP093006271, ISSN: 2405-8440, DOI: 10.1016/j.heliyon.2018.e00668
CHENGHUA LI; WEIDA FENG; LIHUA QIU; CHANGGE XIA; XIURONG SU; CHUNHUA JIN; TINGTING ZHOU; YUAN ZENG; TAIWU LI;: "Characterization of skin ulceration syndrome associated microRNAs in sea cucumberby deep sequencing", FISH & SHELLFISH IMMUNOLOGY, ACADEMIC PRESS, LONDON,, GB, vol. 33, no. 2, 24 April 2012 (2012-04-24), GB , pages 436 - 441, XP028504131, ISSN: 1050-4648, DOI: 10.1016/j.fsi.2012.04.013
ZHOU XIAOXU, CHANG YAQING, ZHAN YAOYAO, WANG XIULI, LIN KAI: "Integrative mRNA-miRNA interaction analysis associate with immune response of sea cucumber Apostichopus japonicus based on transcriptome database", FISH & SHELLFISH IMMUNOLOGY, ACADEMIC PRESS, LONDON,, GB, vol. 72, 1 January 2018 (2018-01-01), GB , pages 69 - 76, XP093006268, ISSN: 1050-4648, DOI: 10.1016/j.fsi.2017.10.031
ZHAI YU, CAO YAN-HUI;ZHANG FENG;CHANG YA-QING: "Screening and Study of miRNA Related to Complement AjC3 in Sea Cucumber Apostichopus Japonicus", JOURNAL OF DALIAN OCEAN UNIVERSITY, vol. 30, no. 6, 31 December 2015 (2015-12-31), pages 585 - 591, XP093006267, ISSN: 2095-1388, DOI: 10.16535/j.cnki.dlhyxb.2015.06.003
GAO KAILUN: "The Expression of Growth-related Genes and Transcriptome Analysis of Individual Size Differences in Apostichopus Japonicus", CHINESE MASTER'S THESES FULL-TEXT DATABASE, AGRICULTURE SCIENCE AND TECHNOLOGY, 2019, NO. 03, PP. D052-65, 15 March 2019 (2019-03-15), XP093006264
FENG WEIDA: "Cloning and Characterization of MicroRNAs from Vibrio Splendidus Challenged Sea Cucumber Apostichopus Japonicus", CHINESE MATER'S THESES FULL-TEXT DATABASE, AGRICULTURE SCIENCE AND TECHNOLOGY, 2014, NO. 08, PP. D052-116, 15 August 2014 (2014-08-15), XP093006262
ZHANG, YI ET AL.: "MiR-210 Regulates Coelomocyte Proliferation through Targeting E2F3 in Apostichopus Japonicus", FISH AND SHELLFISH IMMUNOLOGY, vol. 106, 21 August 2020 (2020-08-21), XP086300604, DOI: 10.1016/j.fsi.2020.08.036
ZHAN, YAOYAO ET AL.: "MicroRNAs Involved in Innate Immunity Regulation in the Sea Cucumber: A Review", FISH AND SHELLFISH IMMUNOLOGY, vol. 95, 25 October 2019 (2019-10-25), XP085933737, DOI: 10.1016/j.fsi.2019.10.049
ZHAN YAOYAO, LI YINGYING, CUI DONGYAO, PEI QIANTONG, SUN JINGXIAN, ZHANG WEIJIE, CHANG YAQING: "Identification and characterization of microRNAs from the tube foot in the sea urchin Strongylocentrotus intermedius", HELIYON, ELSEVIER LTD, GB, vol. 4, no. 6, 1 June 2018 (2018-06-01), GB , pages e00668, XP093006271, ISSN: 2405-8440, DOI: 10.1016/j.heliyon.2018.e00668
CHENGHUA LI; WEIDA FENG; LIHUA QIU; CHANGGE XIA; XIURONG SU; CHUNHUA JIN; TINGTING ZHOU; YUAN ZENG; TAIWU LI;: "Characterization of skin ulceration syndrome associated microRNAs in sea cucumberby deep sequencing", FISH & SHELLFISH IMMUNOLOGY, ACADEMIC PRESS, LONDON,, GB, vol. 33, no. 2, 24 April 2012 (2012-04-24), GB , pages 436 - 441, XP028504131, ISSN: 1050-4648, DOI: 10.1016/j.fsi.2012.04.013
ZHOU XIAOXU, CHANG YAQING, ZHAN YAOYAO, WANG XIULI, LIN KAI: "Integrative mRNA-miRNA interaction analysis associate with immune response of sea cucumber Apostichopus japonicus based on transcriptome database", FISH & SHELLFISH IMMUNOLOGY, ACADEMIC PRESS, LONDON,, GB, vol. 72, 1 January 2018 (2018-01-01), GB , pages 69 - 76, XP093006268, ISSN: 1050-4648, DOI: 10.1016/j.fsi.2017.10.031
ZHAI YU, CAO YAN-HUI;ZHANG FENG;CHANG YA-QING: "Screening and Study of miRNA Related to Complement AjC3 in Sea Cucumber Apostichopus Japonicus", JOURNAL OF DALIAN OCEAN UNIVERSITY, vol. 30, no. 6, 31 December 2015 (2015-12-31), pages 585 - 591, XP093006267, ISSN: 2095-1388, DOI: 10.16535/j.cnki.dlhyxb.2015.06.003
GAO KAILUN: "The Expression of Growth-related Genes and Transcriptome Analysis of Individual Size Differences in Apostichopus Japonicus", CHINESE MASTER'S THESES FULL-TEXT DATABASE, AGRICULTURE SCIENCE AND TECHNOLOGY, 2019, NO. 03, PP. D052-65, 15 March 2019 (2019-03-15), XP093006264
FENG WEIDA: "Cloning and Characterization of MicroRNAs from Vibrio Splendidus Challenged Sea Cucumber Apostichopus Japonicus", CHINESE MATER'S THESES FULL-TEXT DATABASE, AGRICULTURE SCIENCE AND TECHNOLOGY, 2014, NO. 08, PP. D052-116, 15 August 2014 (2014-08-15), XP093006262
Attorney, Agent or Firm:
DALIAN FEIFAN PATENT AGENCY (CN)
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