Title:
MLPA PROBE FOR DETECTING SEPSIS-INDUCING GRAM-NEGATIVE BACTERIA, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/225791
Kind Code:
A1
Abstract:
The present invention relates to a probe for detecting sepsis-inducing gram-negative bacteria, a composition comprising same, a kit, and a method for multi-detecting major gram-negative bacteria from the blood of a sepsis patient by using same. The probe for detecting gram-negative bacteria, the composition, the kit, and the method for detecting major gram-negative bacteria by using same, according to the present invention, reduce a detection time compared to a conventional detection time by optimizing a hybridization time, and increase detection sensitivity by pre-amplifying specific sequences of gram-negative bacteria, so that the existence, type, and drug tolerance of the gram-negative bacteria can be quickly and accurately identified with high sensitivity, without a non-specific reaction with host genome DNA, from a cell-free DNA sample collected from the blood of a patient.
Inventors:
CHUNG YEUN JUN (KR)
LEE DONG GUN (KR)
CHUNG BO RAM (KR)
PARK CHUL MIN (KR)
LEE DONG GUN (KR)
CHUNG BO RAM (KR)
PARK CHUL MIN (KR)
Application Number:
PCT/KR2018/006044
Publication Date:
November 28, 2019
Filing Date:
May 28, 2018
Export Citation:
Assignee:
CATHOLIC UNIV KOREA IND ACADEMIC COOPERATION FOUNDATION (KR)
International Classes:
C12Q1/689; C12Q1/6813; C12Q1/6862
Foreign References:
| KR101838614B1 | 2018-04-26 | |||
| KR20150031735A | 2015-03-25 |
Other References:
PAVLOVIC, M. ET AL.: "Development of a duplex real-time PCR for differentiation between E. coli and Shigella spp", J. APPL. MICROBIOL., vol. 110, no. 5, 14 March 2011 (2011-03-14), pages 1245 - 1251, XP055657107
MARTIN-PENA, R. ET AL.: "Rapid detection of antibiotic resistance in Acinetobacter baumannii using quantitative real-time PCR", J. ANTIMICROB. CHEMOTHER, vol. 68, no. 7, 23 February 2013 (2013-02-23), pages 1572 - 1575, XP055657110
AMIRI, A. ET AL.: "Prevalence of CTX-M-Type and PER Extended-Spectrum beta-Lactamases Among Klebsiella spp. Isolated From Clinical Specimens in the Teaching Hospital of Kashan", IRAN. IRAN. RED CRESCENT MED. J., vol. 18, no. 3, 27 March 2016 (2016-03-27), pages 1 - 7, XP055657115
CHUNG, B. ET AL., MULTIPLEX IDENTIFICATION OF SEPSIS-CAUSING GRAM-NEGATIVE PATHOGENS FROM THE PLASMA OF INFECTED BLOOD, vol. 39, no. 4, 14 December 2017 (2017-12-14), pages 645 - 652
MARTIN-PENA, R. ET AL.: "Rapid detection of antibiotic resistance in Acinetobacter baumannii using quantitative real-time PCR", J. ANTIMICROB. CHEMOTHER, vol. 68, no. 7, 23 February 2013 (2013-02-23), pages 1572 - 1575, XP055657110
AMIRI, A. ET AL.: "Prevalence of CTX-M-Type and PER Extended-Spectrum beta-Lactamases Among Klebsiella spp. Isolated From Clinical Specimens in the Teaching Hospital of Kashan", IRAN. IRAN. RED CRESCENT MED. J., vol. 18, no. 3, 27 March 2016 (2016-03-27), pages 1 - 7, XP055657115
CHUNG, B. ET AL., MULTIPLEX IDENTIFICATION OF SEPSIS-CAUSING GRAM-NEGATIVE PATHOGENS FROM THE PLASMA OF INFECTED BLOOD, vol. 39, no. 4, 14 December 2017 (2017-12-14), pages 645 - 652
Attorney, Agent or Firm:
ERUUM & LEEON INTELLECTUAL PROPERTY LAW FIRM (KR)
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