Description

Technical Field

This invention relates to a mobile handheld device with reusable and replaceable cartridge technology for quantitatively determining an analyte in a fluid sample, and more specifically using a method called Real-time Electrochemical Profiling (REPTM) for detecting biological compounds such as disease protein/genomic biomarkers or chemical agents such as aflatoxins or mycotoxins etc.

Background Art

Biosensors have uses in medical diagnostics, environmental pollutant detection, food and pharmaceutical industries, biological warfare agent detection etc. Devices used for these applications are usually large costly and requires sample transportation to a laboratory. Sometimes procedures include the use of several devices and a central laboratory to get results. These type of analyses may be costly and the quality of the measurement can be affected due to the long distances the sample has to travel, which may have inadequate conditions. This leads to the necessity of onsite detection by means of portable biosensor devices.

The oldest biosensors date back to the 1950s. They were used for oxygen monitoring. By 1980s electrochemical detectors were miniaturized, exhibiting good sensitivity and selectivity. An electrochemical biosensor causes a change in an electrical signal using a specific binding material, such as protein, deoxyribonucleic acid("DNA"), viruses, bacteria, cells, and tissues, and a sensor surface, thereby quantitatively or qualitatively analyzing and testing biomolecules. Detection of a biological material requires a complex process for processing, reaction, and analysis of a reagent. Although the process varies according to an analysis method and the type of the material, a biosensor generally detects a biological material through a complex combination of processes such as filtering, metering, mixing, transport, reaction and washing. Thus, according to conventional art, detection of a biological material is manually performed in respective laboratories using a variety of equipment. Mobile biosensor devices are usually composed of a sensor where the detection - reaction takes place, a transducer (electrochemical, optical or piezoelectric) which translates the signal, an electronic part which processes the signal from the transducer and shows the results to the user.

The mobile hand held device BiSens employs a microfluidic mechanism, bringing an innovative and simple solution with an easy to use cartridge system which includes an on board biosensor and does not need to be calibrated by the user. The aim is to provide mobility and repeatable quality while staying close to the high quality measurements of the laboratories. The cost of the measurement is also reduced significantly while providing constant quality measurement convenience on site.

Disclosure of Invention

The hand-held apparatus consists of a cartridge loading mechanism, a single use all- in-one cartridge with RFID label and a movable syringe with mount for user sample placement, motors and pistons for microfluidic action, a micro potentiostat circuit, RFID reader for cartridge detection, a capacitive touch display , a control unit for signal processing and recording, a battery unit for the power of the overall system and an optional adapter for external power or operation without battery. The device works with temperature compensation and has been calibrated to work between 15°C and 35°C.

The cartridge consists of three stationary syringes and one detachable syringe for sample placement. The biosensor is integrated with the cartridge. The cartridge also houses a waste reservoir. We call this concept an all-in-one cartridge. The fluidics from the syringes, the reaction and waste chamber is connected with microfluidic channels. The fluids are routed from the syringes to the flow cell where the electrodes are housed and then finally end up in the waste chamber.

The integrated biosensor chip consists of specially designed electrodes formed on a glass/silicon oxide substrate (PCT application no: PCT/IB2015/052479). It can detect various analytes based on the bio-recognition elements used for the biological assay. Waste is also integrated on the cartridge. The cartridge is completely sealed including the detachable syringe. When the measurement is started by the user, the syringes are moved by the pistons that are actuated by micro stepper motors. To start a measurement, the user will first place the cartridge in its slot and then select 'start' from the menu on the touchscreen, then the apparatus will read the RFID information of the cartridge and automatically run the embedded protocol. After the measurement is complete, the result is shown to the user on the graphic screen either by means of a plot, in the form of analyte concentration or in the form of yes or no result.

BiSens with its integrated biosensor chip, waste chamber, RFID tag cartridge system will save the user from cleaning procedures as this is done automatically. All the user has to do for a new measurement is to throw away the old cartridge and slot in the new one. The RFID technology integrated into the apparatus makes test preparation obsolete as the protocol is automatically read by the device.

Brief Description of Drawings

The invention is explained in greater detail in the following referred diagram and figures:

Fig. 1 is a perspective view of the apparatus from above.

Fig. 2 is an exploded perspective view of the cartridge, taken from above.

Fig. 3 is an exploded perspective view of the syringe pump mechanism, taken from above.

Fig. 4 is a perspective view of the cartridge head from above.

Fig. 5 is a top view of the cartridge head.

Detailed Description of The Preferred Embodiments

The electrochemical detection apparatus shown in the figures, which includes a set up comprised of an apparatus housing a cartridge, electrical and mechanical syringe pump system, electrochemical biosensor attached to the cartridge, a cartridge comprised of a cartridge body, housing syringes and a fluidic waste deposit, and a cartridge head housing microfluidic channels and a biosensor mount.

Fig. 1 is an overall schematic view of the electrochemical hand held device. A cartridge comprised of two elements; cartridge head(Fig.1-1 ) and cartridge body(Fig.1-2). There is a user detachable syringe(Fig.1-3) for sample placement. The cartridge is placed to its slot(Fig.1 -5) by the user. The cartridge lid(Fig.1-6) is then closed. The protocol is started by the user from the capacitive touchscreen(Fig.1-7). A metal piston(Fig.1-4) injects the appropriate fluid with the help of an electric motor(Fig.3-5). If required the results can be transferred to a PC by the user by means of a USB or network cable using the appropriate ports(Fig.1- 8,9,10). The device also can be integrated to a lab or hospital system if requested by the customer with the help of the Ethernet port(Fig.1-10). If desired the apparatus can be powered on by an external adapter by inserting the power cable into the power socket(Fig.1-11 ).

Fig.2 shows parts of the cartridge. The cartridge is made up of two main pieces; the main cartridge body(Fig.2-1 ) and cartridge head(Fig.2-6). Fig.2-2 is the user fillable syringe with the piston head(Fig.2-7). Fig.2-9 is the syringe sealing ring used between the cartridge head and cartridge main body to prevent any fluidic leak. Fig.2-3 is the sensor slot of the biosensor chip(Fig.2-4). Fig.2-8 is an adhesive stick used to bond the biosensor chip and its cover(Fig.2-5).

Fig.3 is a perspective view from above showing the details of the electro-mechanical piston system. It is configured as a reverse pull-push system. The electrical motor(Fig.3-5) pulls the piece acting as a piston(Fig.3-3). The action of the piston is guided by two metal rods(Fig.3-4) to ensure rigidness of the motion. The pull or push motion is controlled by means of two micro switches(Fig.3-7 and Fig.3-12). The motors are installed on (Fig.3-11 ) and then (Fig.3-11 ) is placed on (Fig.3-13) to install it into the apparatus.

Fig.4 is a detailed perspective view from above of the cartridge head. Fig.4-1 is the cartridge head. (Fig.4-2) is an two sided adhesive sticker to bond the biochip(Fig.4-3) to the cartridge head and biochip cover(Fig.4-4).

Fig.5 is a detailed top view of the cartridge head(Fig.5-6) and the biochip. The microfluidic channels(Fig.5-8) are clearly seen in the transparent drawing of the cartridge head. The fluidics enter the flow cell from the inlet channel(Fig.5-1 ) and leave the flow cell from the outlet channel(Fig.5-5) through the waste deposit opening(Fig.5-7). The syringe head slot(Fig.5-9) on the cartridge head allows for tight mounting of the syringes.