Title:
MODIFICATION OF HEPATITIS B CORE ANTIGEN
Document Type and Number:
WIPO Patent Application WO2001098333
Kind Code:
A3
Abstract:
A protein is provided comprising hepatitis B core antigen (HBcAg) wherein one or more of the four arginine repeats has been deleted, said protein comprising the C-terminal cysteine of HBcAg. The deleted region may be replaced by an epitope from a protein other than HBcAg, in which case the HBcAg acts as a carrier to present the epitope to the immune system. The chimeric protein is useful in prophylactic and therapeutic vaccination of a host, for example against hepatitis B virus.
Inventors:
PAGE MARK (GB)
LI JING-LI (GB)
PUMPENS PAUL (LV)
BORISOVA GALINA (LV)
LI JING-LI (GB)
PUMPENS PAUL (LV)
BORISOVA GALINA (LV)
Application Number:
PCT/GB2001/002817
Publication Date:
March 28, 2002
Filing Date:
June 22, 2001
Export Citation:
Assignee:
CELLTECH PHARMACEUTICALS LTD (GB)
PAGE MARK (GB)
LI JING LI (GB)
PUMPENS PAUL (LV)
BORISOVA GALINA (LV)
PAGE MARK (GB)
LI JING LI (GB)
PUMPENS PAUL (LV)
BORISOVA GALINA (LV)
International Classes:
A61K39/29; A61P1/16; C12N15/09; A61P31/20; C07K14/02; C07K14/47; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P21/02; A61K38/00; A61K48/00; (IPC1-7): C12N15/51; C07K14/02; A61K39/29; A61P31/20
Domestic Patent References:
| WO2000026385A1 | 2000-05-11 |
Foreign References:
| EP0421635A1 | 1991-04-10 |
Other References:
HATTON T ET AL: "RNA AND DNA-BINDING ACTIVITIES IN HEPATITIS B VIRUS CAPSID PROTEIN A MODEL FOR THEIR ROLES IN VIRAL REPLICATION", JOURNAL OF VIROLOGY, vol. 66, no. 9, 1992, pages 5232 - 5241, XP001041852, ISSN: 0022-538X
SCHOEDEL F ET AL: "HYBRID HEPATITIS B VIRUS CORE ANTIGEN AS A VACCINE CARRIER MOIETY: I. PRESENTATION OF FOREIGN EPITOPES", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 44, no. 1/3, January 1996 (1996-01-01), pages 91 - 96, XP000891474, ISSN: 0168-1656
BORISOVA G P ET AL: "RECOMBINANT CORE PARTICLES OF HEPATITIS B VIRUS EXPOSING FOREIGN ANTIGENIC DETERMINANTS ON THEIR SURFACE", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 259, no. 1, 1 December 1989 (1989-12-01), pages 121 - 124, XP002035980, ISSN: 0014-5793
HUI ERIC KA-WAI ET AL: "Hepatitis B virus maturation is affected by the incorporation of core proteins having a C-terminal substitution of arginine or lysine stretches.", JOURNAL OF GENERAL VIROLOGY, vol. 80, no. 10, October 1999 (1999-10-01), pages 2661 - 2671, XP002185072, ISSN: 0022-1317
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1992, ZHOU S ET AL: "CYS RESIDUES OF THE HEPATITIS B VIRUS CAPSID PROTEIN ARE NOT ESSENTIAL FOR THE ASSEMBLY OF VIRAL CORE PARTICLES BUT CAN INFLUENCE THEIR STABILITY", XP002185073, Database accession no. PREV199294115400
SCHOEDEL F ET AL: "HYBRID HEPATITIS B VIRUS CORE ANTIGEN AS A VACCINE CARRIER MOIETY: I. PRESENTATION OF FOREIGN EPITOPES", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 44, no. 1/3, January 1996 (1996-01-01), pages 91 - 96, XP000891474, ISSN: 0168-1656
BORISOVA G P ET AL: "RECOMBINANT CORE PARTICLES OF HEPATITIS B VIRUS EXPOSING FOREIGN ANTIGENIC DETERMINANTS ON THEIR SURFACE", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 259, no. 1, 1 December 1989 (1989-12-01), pages 121 - 124, XP002035980, ISSN: 0014-5793
HUI ERIC KA-WAI ET AL: "Hepatitis B virus maturation is affected by the incorporation of core proteins having a C-terminal substitution of arginine or lysine stretches.", JOURNAL OF GENERAL VIROLOGY, vol. 80, no. 10, October 1999 (1999-10-01), pages 2661 - 2671, XP002185072, ISSN: 0022-1317
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1992, ZHOU S ET AL: "CYS RESIDUES OF THE HEPATITIS B VIRUS CAPSID PROTEIN ARE NOT ESSENTIAL FOR THE ASSEMBLY OF VIRAL CORE PARTICLES BUT CAN INFLUENCE THEIR STABILITY", XP002185073, Database accession no. PREV199294115400
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