Title:
MODIFICATION OF MICROALGAE FOR MAGNETIC PROPERTIES
Document Type and Number:
WIPO Patent Application WO/2012/006423
Kind Code:
A3
Abstract:
Genetically engineered algae strains for biofuels and bioproduct production have improved iron utilization including iron uptake and storage, and exhibit improved growth characteristics, magnetic separation, and magnetic hysteresis induced cell lysis. Pond production algal strain embodiments with high iron scavenging capabilities limit iron availability to contaminating microorganisms and invading species. Accumulation of high iron and other paramagnetic elements content in the form of ferritin enhances the cells magnetic susceptibility improving efficiency of magnetic separation and magnetic hysteresis induced cell lysis. Several genes of the embodiments are capable of improving iron acquisition including the ferritin gene fer1, iron transport gene fea1, and iron reductase gene fre1. Other genes which improve growth in the high iron conditions permitting higher accumulation of iron include radical scavenging enzymes such as superoxide dismutase, peroxidase, catalase, glutathione peroxidase and the Ferritin like DPR/DPS genes which also bind iron and protect DNA from reactive oxygen species.
Inventors:
SAYRE RICHARD (US)
POSTIER BRAD (US)
POSTIER BRAD (US)
Application Number:
PCT/US2011/043192
Publication Date:
March 27, 2014
Filing Date:
July 07, 2011
Export Citation:
Assignee:
PHYCAL LLC (US)
SAYRE RICHARD (US)
POSTIER BRAD (US)
SAYRE RICHARD (US)
POSTIER BRAD (US)
International Classes:
B03C1/02; C12N1/12
Foreign References:
| US20100081130A1 | 2010-04-01 |
Other References:
SCHOLNICK ET AL.: "The Mechanism of Iron Homeostasis in the Unicellular Cyanobacterium Synechocystis sp. PCC 6803 and Its Relationship to Oxidative Stress.", PLANT PHYSIOLOG, vol. 150, 2009, pages 2045 - 2056, XP002631263, DOI: doi:10.1104/PP.109.141853
LONG ET AL.: "FER1 and FER2 Encoding Two Ferritin Complexes in Chlamydomonas reinhardtii Chloroplasts Are Regulated by Iron.", GENETICS, vol. 179, 2008, pages 137 - 147
ALLEN ET AL.: "FEA1, FEA2, and FRE1, Encoding Two Homologous Secreted Proteins and a Candidate Ferrireductase, Are Expressed Coordinately with FOX1 and FTR1 in Iron-Deficient Chlamydomonas reinhardtii.", EUKARYOTIC CELL, vol. 6, no. 10, 2007, pages 1841 - 1852
BUSCH ET AL.: "Ferritin is required for rapid remodeling of the photosynthetic apparatus and minimizes photo-oxidative stress in response to iron availability in Chlamydomonas reinhardtii.", THE PLANT JOURNAL, vol. 55, 2008, pages 201 - 211
CASTRUITA ET AL.: "Overexpression and Characterization of an Iron Storage and DNA-Binding Dps Protein from Trichodesmium erythraeum Iron Storage and DNA-Binding Dps.", APPL. ENVIRON. MICROBIOL., vol. 72, no. 4, 2006, pages 2918 - 2924
DENG ET AL.: "Two Iron-Responsive Promoter Elements Control Expression of FOX1 in Chlamydomonas reinhardtii.", EUKARYOTIC CELL, vol. 6, no. 11, 2007, pages 2163 - 2167
BADARAU ET AL.: "FutA2 Is a Ferric Binding Protein from Synechocystis PCC 6803.", J BIOL CHEM, vol. 283, no. 18, 2008, pages 12520 - 12527
BROWN ET AL.: "A novel cyanobacterium exhibiting an elevated tolerance for iron.", FEMS MICROBIOLOGY ECOLOGY, vol. 52, 2005, pages 307 - 314, XP025292556, DOI: doi:10.1016/j.femsec.2004.11.020
KATOH ET AL.: "Genes Essential to Iron Transport in the Cyanobacterium Synechocystis sp. Strain PCC 6803.", J. BACTERIOL., vol. 183, no. 9, 2001, pages 2779 - 2784
LI ET AL.: "Differential Gene Expression in Response to Hydrogen Peroxide and the Putative PerR Regulon of Synechocystis sp. Strain PCC 6803.", J. BACTERIOL., vol. 186, no. 11, 2004, pages 3331 - 3345
LONG ET AL.: "FER1 and FER2 Encoding Two Ferritin Complexes in Chlamydomonas reinhardtii Chloroplasts Are Regulated by Iron.", GENETICS, vol. 179, 2008, pages 137 - 147
ALLEN ET AL.: "FEA1, FEA2, and FRE1, Encoding Two Homologous Secreted Proteins and a Candidate Ferrireductase, Are Expressed Coordinately with FOX1 and FTR1 in Iron-Deficient Chlamydomonas reinhardtii.", EUKARYOTIC CELL, vol. 6, no. 10, 2007, pages 1841 - 1852
BUSCH ET AL.: "Ferritin is required for rapid remodeling of the photosynthetic apparatus and minimizes photo-oxidative stress in response to iron availability in Chlamydomonas reinhardtii.", THE PLANT JOURNAL, vol. 55, 2008, pages 201 - 211
CASTRUITA ET AL.: "Overexpression and Characterization of an Iron Storage and DNA-Binding Dps Protein from Trichodesmium erythraeum Iron Storage and DNA-Binding Dps.", APPL. ENVIRON. MICROBIOL., vol. 72, no. 4, 2006, pages 2918 - 2924
DENG ET AL.: "Two Iron-Responsive Promoter Elements Control Expression of FOX1 in Chlamydomonas reinhardtii.", EUKARYOTIC CELL, vol. 6, no. 11, 2007, pages 2163 - 2167
BADARAU ET AL.: "FutA2 Is a Ferric Binding Protein from Synechocystis PCC 6803.", J BIOL CHEM, vol. 283, no. 18, 2008, pages 12520 - 12527
BROWN ET AL.: "A novel cyanobacterium exhibiting an elevated tolerance for iron.", FEMS MICROBIOLOGY ECOLOGY, vol. 52, 2005, pages 307 - 314, XP025292556, DOI: doi:10.1016/j.femsec.2004.11.020
KATOH ET AL.: "Genes Essential to Iron Transport in the Cyanobacterium Synechocystis sp. Strain PCC 6803.", J. BACTERIOL., vol. 183, no. 9, 2001, pages 2779 - 2784
LI ET AL.: "Differential Gene Expression in Response to Hydrogen Peroxide and the Putative PerR Regulon of Synechocystis sp. Strain PCC 6803.", J. BACTERIOL., vol. 186, no. 11, 2004, pages 3331 - 3345
Attorney, Agent or Firm:
HUDZINSKi, Michael E. (925 Euclid Avenue1150 Huntington Buildin, Cleveland Ohio, US)
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